公开(公告)号：US20210088511A1

公开(公告)日：2021-03-25

申请号：US17039245

申请日：2020-09-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Peter CRISALLI ,  Dmitriy GREMYACHINSKIY ,  Dieter HEINDL ,  Hannes KUCHELMEISTER ,  Michael SCHRAEML ,  Andrew TRANS

IPC: G01N33/543 ,  G01N33/487

Abstract: Provided are nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution. The compositions include an analyte detection complex that is associated with a nanopore to form a nanopore assembly, the analyte detection complex including an analyte ligand. As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte. By comparing the total number of analyte-ligand binding pairs to a control binding count, the concentration of the analyte can be determined. In other examples, further increasing the second voltage can result in dissociation of the analyte-ligand pair, from which a dissociation voltage—and hence a dissociation constant—can be determined.