公开(公告)号：US20220404347A1

公开(公告)日：2022-12-22

申请号：US17350822

申请日：2021-06-17

Applicant: Roche Diagnostics Operations, Inc. ,  Roche Sequencing Solutions, Inc.

Inventor： Yann ASTIER ,  Dieter HEINDL ,  Hannes KUCHELMEISTER ,  Nikolaus-Peter STENGELE

IPC: G01N33/543 ,  C12N15/115 ,  C12N15/10

Abstract: The present invention relates to diagnostic test and technology. In particular, it relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with at least one sensor element comprising at least one binding agent which is capable of specifically binding to the analyte and which comprises at least one magnetic label; and in functional proximity thereto a magnetic tunnel junction generating a signal which is altered upon binding of the analyte to the binding agent for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the at least one binding agent, measuring an altered signal generated by the magnetic tunnel junction upon analyte binding to the at least one binding agent comprising the at least one magnetic label, and determining the analyte based on the altered signal which is generated by the magnetic tunnel junction. The present invention further relates to a device for determining an analyte suspected to be present in a sample and for using such a device. Moreover, the present invention furthermore relates to an aptamer which is capable of specifically binding to an analyte and which comprises at least one magnetic label and a method for identifying such an aptamer. Finally, the invention relates to a kit for determining an analyte suspected to be present in a sample.

公开(公告)号：US20210088511A1

公开(公告)日：2021-03-25

申请号：US17039245

申请日：2020-09-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Peter CRISALLI ,  Dmitriy GREMYACHINSKIY ,  Dieter HEINDL ,  Hannes KUCHELMEISTER ,  Michael SCHRAEML ,  Andrew TRANS

IPC: G01N33/543 ,  G01N33/487

Abstract: Provided are nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution. The compositions include an analyte detection complex that is associated with a nanopore to form a nanopore assembly, the analyte detection complex including an analyte ligand. As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte. By comparing the total number of analyte-ligand binding pairs to a control binding count, the concentration of the analyte can be determined. In other examples, further increasing the second voltage can result in dissociation of the analyte-ligand pair, from which a dissociation voltage—and hence a dissociation constant—can be determined.