公开(公告)号：US20240377388A1

公开(公告)日：2024-11-14

申请号：US18669957

申请日：2024-05-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael GERG ,  Dieter HEINDL ,  Lars HILLRINGHAUS ,  Klaus HIRZEL ,  Caroline Dorothea HOJER ,  Florian HUBER ,  Hans-Peter JOSEL ,  Thomas MEIER ,  Michael SCHRAEML ,  Edgar VOSS

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US20220107310A1

公开(公告)日：2022-04-07

申请号：US17551723

申请日：2021-12-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael GERG ,  Dieter HEINDL ,  Lars HILLRINGHAUS ,  Klaus HIRZEL ,  Caroline Dorothea HOJER ,  Florian HUBER ,  Hans-Peter JOSEL ,  Thomas MEIER ,  Michael SCHRAEML ,  Edgar VOSS

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.