公开(公告)号：US20220195497A1

公开(公告)日：2022-06-23

申请号：US17544110

申请日：2021-12-07

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank BERGMANN ,  Dieter HEINDL ,  Michael SCHRAEML ,  JOHANNES STOECKEL

IPC: C12Q1/6816

Abstract: The present report relates to hybridizing single-stranded (ss-) oligonucleotides which entirely consist of locked nucleic acid (LNA) monomers. The present document shows hybridization experiments with pairs of entirely complementary ss-oligonucleotides which fail to form a duplex within a given time interval. The present report provides methods to identify such incompatible oligonucleotide pairs. In another aspect, the present report provides pairs of complementary ss-oligonucleotides which are capable of rapid duplex formation. The present report also provides methods to identify and select compatible oligonucleotide pairs. In yet another aspect the present report provides use of compatible oligonucleotide pairs as binding partners in binding assays, e.g. receptor-based assays.