公开(公告)号：US20220195497A1

公开(公告)日：2022-06-23

申请号：US17544110

申请日：2021-12-07

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank BERGMANN ,  Dieter HEINDL ,  Michael SCHRAEML ,  JOHANNES STOECKEL

IPC: C12Q1/6816

Abstract: The present report relates to hybridizing single-stranded (ss-) oligonucleotides which entirely consist of locked nucleic acid (LNA) monomers. The present document shows hybridization experiments with pairs of entirely complementary ss-oligonucleotides which fail to form a duplex within a given time interval. The present report provides methods to identify such incompatible oligonucleotide pairs. In another aspect, the present report provides pairs of complementary ss-oligonucleotides which are capable of rapid duplex formation. The present report also provides methods to identify and select compatible oligonucleotide pairs. In yet another aspect the present report provides use of compatible oligonucleotide pairs as binding partners in binding assays, e.g. receptor-based assays.

公开(公告)号：US20220011301A1

公开(公告)日：2022-01-13

申请号：US17292660

申请日：2019-11-14

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank BERGMANN ,  Dieter HEINDL ,  Tobias OELSCHLAEGEL ,  Johannes STOECKEL

IPC: G01N33/543

Abstract: The present disclosure relates to a solid phase coated with (strept)avidin and having attached thereto, by way of biotin:(strept)avidin interaction, a biotinylated first member of a binding pair, wherein the attached first member is capable of binding to a second member of the binding pair, but is not capable of binding to biotin or to (strept)avidin, and wherein no member of the binding pair is capable of hybridizing with a naturally-occurring single-stranded nucleic acid. The solid phase is particularly useful in immunoassays with samples having high content in biotin or (strept)avidin-binding derivatives thereof. The present disclosure further provides uses, kits and methods, particularly for determination of an analyte in a sample.

公开(公告)号：US20220064212A1

公开(公告)日：2022-03-03

申请号：US17419073

申请日：2020-01-03

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank BERGMANN ,  Sebastian Johannes POMPLUN

IPC: C07K1/20

Abstract: The present disclosure relates to the use of a capping and capture reagent in solid phase peptide synthesis. The present disclosure further relates to a method of solid phase peptide synthesis, wherein a capping and capture reagent according to the present disclosure is used. The present disclosure further relates to a method for purification of a (full-length) synthetic peptide via use of a capping and capture reagent according to the present disclosure. The present disclosure also relates to a kit comprising a capping and capture reagent according to the present disclosure and an amino oxy resin or a hydrazine resin and the use of the kit.