公开(公告)号：US09341552B2

公开(公告)日：2016-05-17

申请号：US14575423

申请日：2014-12-18

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Apostolos Kyriatsoulis ,  Susanne Feldmann ,  Eramus Huber ,  Nicole Horn ,  Leopold von Proff ,  Uwe Kobold ,  Angela Puhlmann

IPC: G01N33/82 ,  G01N1/34 ,  C07K16/44 ,  G01N33/52

CPC classification number: G01N1/34 ,  C07K16/44 ,  G01N33/52 ,  G01N33/82 ,  Y10T436/10

Abstract: The present invention concerns a reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, a method for the detection of a 25-hydroxyvitamin D compound in which the 25-hydroxyvitamin D compound is released from vitamin D-binding protein using this reagent and the mixture obtained in this manner is analyzed, the use of the reagent to release vitamin D compounds as well as a kit for detecting 25-hydroxyvitamin D which contains the reagent for releasing vitamin D compounds in addition to the usual immunological reagents.

Abstract translation: 本发明涉及用于释放与维生素D结合蛋白结合的维生素D化合物的试剂组合物，使用该维生素D结合蛋白从维生素D结合蛋白中释放25-羟基维生素D化合物的方法 试剂和以这种方式获得的混合物，使用试剂释放维生素D化合物以及用于检测25-羟基维生素D的试剂盒，其中含有用于释放维生素D化合物的试剂以及通常的免疫试剂。

公开(公告)号：US20240400672A1

公开(公告)日：2024-12-05

申请号：US18662694

申请日：2024-05-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Hans-Peter Josel ,  Ute Jucknischke ,  Holger Lehmann ,  Anastasia Markina ,  Giuseppe Prencipe ,  Leopold von Proff ,  Michael Schraeml ,  Steinar Halldórsson

IPC: C07K16/26 ,  G01N33/78

Abstract: The present invention provides a novel monoclonal antibody specifically binding to L-Thyroxine (T4) and compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such monoclonal antibodies, host cells expressing said antibodies, methods of producing such antibodies and diagnostic methods using such monoclonal antibodies. The monoclonal antibody of the invention comprises a heavy chain variable domain (VH) comprising V or A in position 33; Y in position 50; W in position 52; I in position 98, G, A or V in position 99; Y in position 100; and I in position 100b; and a light chain variable domain (VL) comprising amino acids H or Y in position 28; N or K in position 29; W in position 32; G or A in position 91; Y, W or F in position 92; S or T in position 93; Y or F in position 95b; N, S, T or Q in position 95c; and H in position 96, wherein the positions of the amino acids in the VH and the VL are indicated according to the Kabat numbering scheme, respectively.

公开(公告)号：US20150104876A1

公开(公告)日：2015-04-16

申请号：US14575423

申请日：2014-12-18

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Apostolos Kyriatsoulis ,  Susanne Feldmann ,  Eramus Huber ,  Nicole Horn ,  Leopold von Proff ,  Uwe Kobold ,  Angela Puhlmann

IPC: G01N1/34

CPC classification number: G01N1/34 ,  C07K16/44 ,  G01N33/52 ,  G01N33/82 ,  Y10T436/10

Abstract: The present invention concerns a reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, a method for the detection of a 25-hydroxyvitamin D compound in which the 25-hydroxyvitamin D compound is released from vitamin D-binding protein using this reagent and the mixture obtained in this manner is analyzed, the use of the reagent to release vitamin D compounds as well as a kit for detecting 25-hydroxyvitamin D which contains the reagent for releasing vitamin D compounds in addition to the usual immunological reagents.