公开(公告)号：US20190250158A1

公开(公告)日：2019-08-15

申请号：US16389066

申请日：2019-04-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Uwe Kobold ,  Robert Lang ,  Andreas Leinenbach

IPC: G01N33/566 ,  G01N33/573 ,  G01N33/68

CPC classification number: G01N33/566 ,  G01N33/543 ,  G01N33/573 ,  G01N33/6848

Abstract: The present disclosure relates to a method for detecting an analyte involving a) contacting the analyte, a capture agent and a solid surface to form a capture complex attached to a solid surface, b) cleaving at least one covalent bond within the analyte and/or within said capture agent in the capture complex and, thereby, releasing the analyte or a fragment thereof from the capture complex, and c) detecting at least one fragment of the analyte and, thereby, detecting the analyte. The present disclosure further relates to a kit having i) a capture agent binding to an analyte; and ii) an agent cleaving at least one covalent bond within the analyte and/or within the capture agent; and to the use of a protease for releasing fragments of an analyte for detecting the analyte, wherein the analyte is bound to a solid surface via a capture agent.

公开(公告)号：US11585811B2

公开(公告)日：2023-02-21

申请号：US16389066

申请日：2019-04-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Uwe Kobold ,  Robert Lang ,  Andreas Leinenbach

IPC: G01N33/53 ,  G01N33/566 ,  G01N33/68 ,  G01N33/543 ,  G01N33/573

Abstract: A method for detecting an analyte involving a) contacting the analyte, a capture agent and a solid surface to form a capture complex attached to a solid surface, b) cleaving at least one covalent bond within the analyte and/or within said capture agent in the capture complex and, thereby, releasing the analyte or a fragment thereof from the capture complex, and c) detecting at least one fragment of the analyte and, thereby, detecting the analyte is disclosed herein. A kit having i) a capture agent binding to an analyte; and ii) an agent cleaving at least one covalent bond within the analyte and/or within the capture agent; and to the use of a protease for releasing fragments of an analyte for detecting the analyte, wherein the analyte is bound to a solid surface via a capture agent is also disclosed herein.

公开(公告)号：US20240385155A1

公开(公告)日：2024-11-21

申请号：US18694492

申请日：2022-09-22

Applicant: Roche Diagnostics Operations, Inc. ,  Hitachi High-Tech Corporation

Inventor： Robert Lang ,  Indranil Mitra ,  Yuichiro Hashimoto ,  Yuka Sugawara

IPC: G01N30/86 ,  G01N30/02 ,  G01N30/32 ,  G01N30/72

Abstract: An analytical system comprising a mass spectrometer and an ionization source. The analytical system further comprises an analytical fluidic system connectable to the ionization source for infusing samples into the mass spectrometer via the ionization source, a downstream pump fluidically connectable to the ionization source via the downstream valve, where the downstream pump is fluidically connected to a plurality of fluid containers comprising respective fluids, the fluids comprising a concentrated composition for calibrating the mass spectrometer and at least one diluent for diluting the at least one concentrated composition. The analytical system further comprises a controller configured to control the downstream pump to obtain at least one diluted composition by automatically mixing a concentrated composition with a diluent with a predetermined dilution factor, to infuse the diluted composition into the ionization source, to obtain a mass spectrum of the diluted composition and to execute a calibration of the mass spectrometer. A respective automated analytical method.

公开(公告)号：US11581178B2

公开(公告)日：2023-02-14

申请号：US17453683

申请日：2021-11-05

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Lang ,  Stefan Quint ,  Gabriele Punzmann

IPC: H01J49/00 ,  H01J49/04 ,  G01N30/72 ,  H01J49/02 ,  H01J49/16

Abstract: A mass spectrometry (MS) apparatus is provided. The MS apparatus includes a mass spectrometer, an ionization source coupled to the mass spectrometer, and a flow injection system (FIS) coupled to the ionization source. The ionization source is configured to provide an ionized gas flow of an analyte towards an entrance of the mass spectrometer. The ionization source is further configured to provide a second gas flow of a second gas. The MS apparatus is configured to measure a mass spectrometer (MS) signal of the analyte. The MS apparatus is further configured to analyze a dependency of the MS signal of the analyte versus a parameter of the second gas flow or a state of the second gas flow and to determine a condition of the apparatus based on the analyzed dependency.

公开(公告)号：US20240385154A1

公开(公告)日：2024-11-21

申请号：US18689808

申请日：2022-09-05

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Kirill Tarasov ,  Andreas Reichert ,  Manuela Hummel ,  Robert Lang ,  Marius Wagner

IPC: G01N30/86 ,  G01N30/02 ,  G01N30/72

Abstract: A computer implemented method for automated quality check of chromatographic and/or mass spectral data is disclosed. The method comprises the following steps: a) (110) providing processed chromatographic and/or mass spectral data obtained by at least one mass spectrometry device (112); b) (114) classifying quality of the chromatographic and/or mass spectral data by applying at least one trained machine learning model on the chromatographic and/or mass spectral data, wherein the trained machine learning model uses at least one regression model (116), wherein the trained machine learning model is trained on at least one training dataset comprising historical and/or semi-synthetic chromatographic and/or mass spectral data, wherein the trained machine learning model is an analyte-specific trained machine learning model.

公开(公告)号：US20220165557A1

公开(公告)日：2022-05-26

申请号：US17453683

申请日：2021-11-05

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Lang ,  Stefan Quint ,  Gabriele Punzmann

IPC: H01J49/04 ,  H01J49/02 ,  H01J49/16 ,  G01N30/72

Abstract: A mass spectrometry (MS) apparatus is provided. The MS apparatus includes a mass spectrometer, an ionization source coupled to the mass spectrometer, and a flow injection system (FIS) coupled to the ionization source. The ionization source is configured to provide an ionized gas flow of an analyte towards an entrance of the mass spectrometer. The ionization source is further configured to provide a second gas flow of a second gas. The MS apparatus is configured to measure a mass spectrometer (MS) signal of the analyte. The MS apparatus is further configured to analyze a dependency of the MS signal of the analyte versus a parameter of the second gas flow or a state of the second gas flow and to determine a condition of the apparatus based on the analyzed dependency.