公开(公告)号：US20090233287A1

公开(公告)日：2009-09-17

申请号：US12298191

申请日：2007-04-25

申请人： Roelof Ary Lans Bovenberg ,  Marco Alexander Van den Berg ,  Susanne Hage ,  Paul Klaassen ,  Bernard Meijrink ,  Lourina Madeleine Raamsdonk

发明人： Roelof Ary Lans Bovenberg ,  Marco Alexander Van den Berg ,  Susanne Hage ,  Paul Klaassen ,  Bernard Meijrink ,  Lourina Madeleine Raamsdonk

IPC分类号： C12Q1/68 ,  C12N1/19 ,  C12P1/00 ,  C12P17/06 ,  C12P17/04 ,  C12P7/02 ,  C12P17/16 ,  C12P7/42

CPC分类号： C12N9/1029 ,  C12N9/0004 ,  C12N9/93 ,  C12P7/02 ,  C12P13/02 ,  C12P17/06 ,  C12P17/181 ,  C12P17/182 ,  C12P17/188

摘要： The present invention provides a recombinant Penicillium chrysogenum strain characterized in that a gene selected from the list consisting of penDE, pcbAB and pcbC is inactivated and the use of such a strain for the preparation of a compound of interest. Furthermore, it is an object of the present invention to provide a method for the production of a compound of interest in a eukaryotic recombinant microorganism comprising the steps of: (a) Reducing the level of secondary metabolite production in said microorganism with 50-100%; (b) Introducing a heterologous gene into said microorganism

摘要翻译： 本发明提供重组产黄青霉菌株，其特征在于，从PenDE，pcbAB和pcbC列表中选出的基因被灭活，并且使用这样的菌株来制备感兴趣的化合物。 此外，本发明的目的是提供一种在真核重组微生物中生产感兴趣的化合物的方法，包括以下步骤：（a）降低所述微生物中次级代谢产物的产生水平，其中50-100％ ; （b）将异源基因引入所述微生物