公开(公告)号：US20100129387A1

公开(公告)日：2010-05-27

申请号：US12295198

申请日：2007-03-29

申请人： Rolando Pajón Feyt ,  Karem Cobas Acosta ,  Yasser Perera Negrin ,  Gretel Sardiñas Garcia ,  Darién Garcia Diaz ,  Sonia Gonzalez Blanco

发明人： Rolando Pajón Feyt ,  Karem Cobas Acosta ,  Yasser Perera Negrin ,  Gretel Sardiñas Garcia ,  Darién Garcia Diaz ,  Sonia Gonzalez Blanco

IPC分类号： A61K39/00 ,  C07K14/00 ,  G01N33/53 ,  A61P31/04 ,  A61P37/04

CPC分类号： A61K38/16 ,  A61K39/095 ,  C07K14/22 ,  Y10T436/143333

摘要： The present invention is related to field of medicine, particularly to the development of pharmaceutical formulation containing NMB0606 protein. Formulation described in this invention is able to confer protection against different diseases caused or not by pathogenic agents. NMB0606 protein was identified as a Neisseria meningitidis outer membrane vesicle (OMV) component, and it was obtained through recombinant DNA technology being its immunogenicity and protective activity evaluated in animal models. Due to the high level of conservation the NMB0606 coding gene has shown, pharmaceutical composition containing this protein has a high value as inducers of a cross-reactive immune response. Formulation presented in this invention is applicable to the field of human medicine.

摘要翻译： 本发明涉及药物领域，特别涉及含有NMB0606蛋白质的药物制剂的开发。 本发明描述的制剂能够赋予针对由病原体引起或不由病原体引起的不同疾病的保护作用。 NMB0606蛋白被鉴定为脑膜炎奈瑟氏球菌外膜囊泡（OMV）组分，通过重组DNA技术获得其在动物模型中评估的免疫原性和保护活性。 由于NMB0606编码基因的高度保守性，含有这种蛋白质的药物组合物作为交叉反应性免疫反应的诱导剂具有很高的价值。 本发明提出的制剂适用于人类医学领域。

公开(公告)号：US07244411B2

公开(公告)日：2007-07-17

申请号：US10988943

申请日：2004-11-15

申请人： Lázaro Hiram Betancourt Núñez ,  Jorge Fernández de Cossio Dorta-Duque ,  Vladimir Armando Besada Pérez ,  Jeovanis Gil Valdés ,  Luis Javier González López ,  Gabriel Ramón Padrón Palomares ,  Rolando Pajón Feyt ,  Félix Modesto Álvarez Gil

发明人： Lázaro Hiram Betancourt Núñez ,  Jorge Fernández de Cossio Dorta-Duque ,  Vladimir Armando Besada Pérez ,  Jeovanis Gil Valdés ,  Luis Javier González López ,  Gabriel Ramón Padrón Palomares ,  Rolando Pajón Feyt ,  Félix Modesto Álvarez Gil

IPC分类号： A61K51/00 ,  A61M36/14

CPC分类号： G01N33/6848 ,  C12Q1/37

摘要： The present invention describes a method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixture. The method comprises the selective isolation from every protein of those peptides that neither contain arginine nor histidine (NHNR peptides), and the determination of the relative concentrations of one or several proteins in different samples from the ratio between the areas of the estimated theoretical spectra for the NHNR peptides labeled with different isotopes in each sample. The determination of the relative concentration of proteins is valid for any type of isotopic label of the NHNR peptides. The method avoids the separation and purification of the proteins present in a complex mixture, and the analysis of all peptides generated from the enzymatic digest of the samples. The method is applicable to the identification of proteins with vacunal, therapeutic and diagnostic aims.

摘要翻译： 本发明描述了用于鉴定和定量分析复合物中蛋白质的选择性肽分离方法。 该方法包括从不含精氨酸和组氨酸（NHNR肽）的那些肽的每种蛋白质的选择性分离，以及将不同样品中一种或几种蛋白质的相对浓度与估计的理论光谱面积之比 在每个样品中用不同同位素标记的NHNR肽。 蛋白质的相对浓度的测定对于NHNR肽的任何类型的同位素标记是有效的。 该方法避免了复杂混合物中存在的蛋白质的分离和纯化，以及从样品的酶促消化产生的所有肽的分析。 该方法适用于鉴定具有病毒，治疗和诊断目的的蛋白质。