公开(公告)号：US20140227305A1

公开(公告)日：2014-08-14

申请号：US13818711

申请日：2011-08-25

申请人： Romana Lange-Ruiss ,  Gilbert Reisbach ,  Wolfgang Hammerschmidt ,  Reinhard Zeidler

发明人： Romana Lange-Ruiss ,  Gilbert Reisbach ,  Wolfgang Hammerschmidt ,  Reinhard Zeidler

IPC分类号： A61K39/245

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/57 ,  A61K2039/575 ,  C07K14/005 ,  C07K2317/569 ,  C12N7/00 ,  C12N2710/16223 ,  C12N2710/16234 ,  C12N2710/16252 ,  C12N2710/16262 ,  C12N2710/16271

摘要： The present invention relates to a vaccine comprising a particle, said particle comprising (i) at least one Epstein-Barr virus (EBV) structural polypeptide, (ii) at least one EBV lytic polypeptide, (iii) membrane lipids, said particle being devoid of EBV DNA, wherein (a) the B-cell transformation capacity of one or more EBV polypeptides required for B-cell transformation as comprised in said particle is disabled while their immunogenicity is maintained; and/or (b) said particle is devoid of one or more EBV polypeptides required for B-cell transformation. Furthermore, the invention relates to a method for generating a particle, to a cell obtained in the method of the invention, a kit comprising the vaccine or the particle generated according to the method of the invention. Also, the invention relates to the use of the vaccine or the particle generated according to the method of the invention for generating CD8+ cells specific for an EBV antigen.

摘要翻译： 本发明涉及包含颗粒的疫苗，所述颗粒包含（i）至少一种爱泼斯坦 - 巴尔病毒（EBV）结构多肽，（ii）至少一种EBV溶解多肽，（iii）膜脂质，所述颗粒无缺陷 的EBV DNA，其中（a）包含在所述颗粒中的B细胞转化所需的一种或多种EBV多肽的B细胞转化能力在其免疫原性保持时被禁用; 和/或（b）所述颗粒缺乏B细胞转化所需的一种或多种EBV多肽。 此外，本发明涉及一种生成颗粒的方法，本发明方法获得的细胞包含根据本发明方法生成的疫苗或颗粒的试剂盒。 此外，本发明涉及根据本发明的方法产生的疫苗或颗粒用于产生对EBV抗原特异性的CD8 +细胞的用途。

公开(公告)号：US06210959B1

公开(公告)日：2001-04-03

申请号：US09323678

申请日：1999-06-02

申请人： Antal Lodri ,  Jean-Pierre Kremer ,  Gilbert Reisbach ,  Guoquing Liu

发明人： Antal Lodri ,  Jean-Pierre Kremer ,  Gilbert Reisbach ,  Guoquing Liu

IPC分类号： C12M112

CPC分类号： C12M23/08 ,  C12M23/34 ,  C12M35/08

摘要： For avoidance of cell aggregation and damage to the cells as well as for a reduction of the risk of contamination, an apparatus for the cultivation and concentration of non-adherent cells as well as for the co-culture of two different cell species consists of an elongate hollow vessel comprising a resting surface extending in parallel with the longitudinal axis of said vessel for positioning the hollow vessel for cell culture operation, a standing surface provided orthogonally on the longitudinal axis for positioning the hollow vessel for cell-harvesting operation or for the co-culture of two cell species, at least one closable charging and discharging opening formed on the hollow vessel on the section of the latter which is opposite to said standing surface, and a partitioning wall extending inside the hollow vessel from the standing surface thereof, which partly subdivides the interior space of the vessel, with this partitioning wall being formed at least in parts with a liquid-permeable, however cell-impermeable micro-porous membrane or a cell-impermeable screen, respectively, in the region near the standing surface.

摘要翻译： 为了避免细胞聚集和细胞损伤以及降低污染风险，用于培养和浓缩非贴壁细胞以及用于两种不同细胞种类共同培养的装置包括： 细长的中空容器包括与所述容器的纵向轴线平行延伸的搁置表面，用于定位用于细胞培养操作的中空容器，在纵轴线上正交设置的定位表面，用于定位用于细胞收集操作的中空容器或用于共同作用 - 两种细胞种类的培养，至少一个在所述中空容器上的与所述站立表面相对的部分上的中空容器上形成的可关闭的充填和排出口，以及从所述中空容器的直立表面延伸到所述中空容器内的分隔壁， 部分地细分容器的内部空间，该分隔壁至少部分地形成有液体渗透 分别在立体表面附近的区域中的细胞不渗透的微孔膜或细胞不可渗透的屏幕。