利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

7 条记录 (耗时 0.023 秒)

    EPSTEIN-BARR-VIRUS VACCINE
    1.
    发明申请
    EPSTEIN-BARR-VIRUS VACCINE 审中-公开

    公开(公告)号：US20140227305A1

    公开(公告)日：2014-08-14

    申请号：US13818711

    申请日：2011-08-25

    申请人： Romana Lange-Ruiss ,  Gilbert Reisbach ,  Wolfgang Hammerschmidt ,  Reinhard Zeidler

    发明人： Romana Lange-Ruiss ,  Gilbert Reisbach ,  Wolfgang Hammerschmidt ,  Reinhard Zeidler

    IPC分类号： A61K39/245

    CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/57 ,  A61K2039/575 ,  C07K14/005 ,  C07K2317/569 ,  C12N7/00 ,  C12N2710/16223 ,  C12N2710/16234 ,  C12N2710/16252 ,  C12N2710/16262 ,  C12N2710/16271

    摘要： The present invention relates to a vaccine comprising a particle, said particle comprising (i) at least one Epstein-Barr virus (EBV) structural polypeptide, (ii) at least one EBV lytic polypeptide, (iii) membrane lipids, said particle being devoid of EBV DNA, wherein (a) the B-cell transformation capacity of one or more EBV polypeptides required for B-cell transformation as comprised in said particle is disabled while their immunogenicity is maintained; and/or (b) said particle is devoid of one or more EBV polypeptides required for B-cell transformation. Furthermore, the invention relates to a method for generating a particle, to a cell obtained in the method of the invention, a kit comprising the vaccine or the particle generated according to the method of the invention. Also, the invention relates to the use of the vaccine or the particle generated according to the method of the invention for generating CD8+ cells specific for an EBV antigen.

    摘要翻译： 本发明涉及包含颗粒的疫苗，所述颗粒包含（i）至少一种爱泼斯坦 - 巴尔病毒（EBV）结构多肽，（ii）至少一种EBV溶解多肽，（iii）膜脂质，所述颗粒无缺陷 的EBV DNA，其中（a）包含在所述颗粒中的B细胞转化所需的一种或多种EBV多肽的B细胞转化能力在其免疫原性保持时被禁用; 和/或（b）所述颗粒缺乏B细胞转化所需的一种或多种EBV多肽。 此外，本发明涉及一种生成颗粒的方法，本发明方法获得的细胞包含根据本发明方法生成的疫苗或颗粒的试剂盒。 此外，本发明涉及根据本发明的方法产生的疫苗或颗粒用于产生对EBV抗原特异性的CD8 +细胞的用途。

    Method for the stimulation of T cells having a desired antigen specificity
    6.
    发明授权
    Method for the stimulation of T cells having a desired antigen specificity 失效
    刺激具有所需抗原特异性的T细胞的方法

    公开(公告)号：US06194205B1

    公开(公告)日：2001-02-27

    申请号：US09152653

    申请日：1998-09-14

    申请人： Martin Staege ,  Bettina Kempkes ,  Georg W. Bornkamm ,  Wolfgang Hammerschmidt ,  Ursula Zimber-Strobl ,  Axel Polack

    发明人： Martin Staege ,  Bettina Kempkes ,  Georg W. Bornkamm ,  Wolfgang Hammerschmidt ,  Ursula Zimber-Strobl ,  Axel Polack

    IPC分类号： C12N510

    CPC分类号： C12N5/0636 ,  A61K2039/5158 ,  C12N2501/52 ,  C12N2502/11 ,  C12N2510/04

    摘要： T cells having a desired antigen specificity are stimulated by (a) introducing immortalizing genes into antigen-presenting cells in a manner permitting regulation of the expression and/or function of at least one of these genes to achieve conditionally immortalized antigen-presenting cells; (b) introducing a gene encoding the desired antigen into the immortalized cells in a manner permitting the antigen to be expressed after the expression and/or abolishment of the function of at least one of the immortalizing genes stops; (c) expanding the immortalized antigen-presenting cells by expression and/or functional activation of the immortalizing genes; (d) completing the proliferation of the immortalized antigen-presenting cells by stopping the expression and/or abolishing the function of at least one of the controllable immortalizing genes; (e) continuing the expression of the antigen; (f) adding leucocytic cells including T cells and cultivating the cell mixture to stimulate the T cells directed against the desired antigen; and (g) optionally purifying and isolating the stimulated T cells.

    摘要翻译： 通过（a）以允许调节这些基因中的至少一个的表达和/或功能的方式将永生化基因引入抗原呈递细胞来刺激具有所需抗原特异性的T细胞，以获得条件永生化的抗原呈递细胞; （b）以表达和/或消除至少一种永生化基因的功能停止后，以允许抗原表达的方式将编码所需抗原的基因导入永生化细胞; （c）通过永生化基因的表达和/或功能活化来扩增永生化的抗原呈递细胞; （d）通过停止表达和/或消除至少一种可控永生化基因的功能来完成永生化抗原呈递细胞的增殖; （e）继续表达抗原; （f）加入包含T细胞的白细胞，培养细胞混合物以刺激针对所需抗原的T细胞; 和（g）任选地纯化和分离受刺激的T细胞。

    Lytic origin of replication for Epstein-Barr virus (EBV)
    7.
    发明授权
    Lytic origin of replication for Epstein-Barr virus (EBV) 失效
    爱泼斯坦 - 巴尔病毒（EBV）的复制起始点

    公开(公告)号：US5194601A

    公开(公告)日：1993-03-16

    申请号：US409843

    申请日：1989-09-18

    申请人： William M. Sugden ,  Wolfgang Hammerschmidt

    发明人： William M. Sugden ,  Wolfgang Hammerschmidt

    IPC分类号： C12N15/38 ,  C12N15/869

    CPC分类号： C12N15/86 ,  C12N2710/16243

    摘要： The invention provides lymphotrophic herpes virus (preferably EBV) recombinants. The portions of the virus that are responsible for the packaging and lytic phase replication have been isolated and cloned. When used with vectors and hosts containing a segment that controls plasmid replication, they provide a means of carrying foreign genes into B-lymphocytes.

    摘要翻译： 本发明提供淋巴细胞性疱疹病毒（优选EBV）重组体。 负责包装和裂解相复制的病毒部分已被分离并克隆。 当与含有控制质粒复制的区段的载体和宿主一起使用时，它们提供将外来基因携带到B淋巴细胞中的方法。