-
公开(公告)号：US20100021965A1
公开(公告)日：2010-01-28
申请号：US12083535
申请日：2006-10-26
申请人： Ulrich Goepfert , Erhard Kopetzki
发明人： Ulrich Goepfert , Erhard Kopetzki
CPC分类号： C12N15/85 , C12N7/00 , C12N2710/16252 , C12N2800/108
摘要： A method is described for the expression of heterologous polypeptides in rodent cells. The method comprises the oriP/EBNA-1 episomal replication and maintenance system of the Epstein-Barr-Virus (EBV). With the stable integration of an EBNA-1-protein expression cassette under the control of a promoter into the genome of a rodent cell an EBNA-1-protein expression in the cells was obtained. The heterologous protein is expressed from an episome comprising an EBV origin of replication and a functional expression cassette of said heterologous protein. The invention further comprises transformed rodent cell lines, a method for the production of a heterologous protein in said cell lines and a kit for the construction of said cell lines.
摘要翻译： 描述了在啮齿动物细胞中表达异源多肽的方法。 该方法包括爱泼斯坦 - 巴尔病毒（EBV）的oriP / EBNA-1附加体复制和维持系统。 通过将启动子控制下的EBNA-1蛋白表达盒稳定整合到啮齿动物细胞的基因组中，获得细胞中的EBNA-1蛋白表达。 异源蛋白质由包含EBV复制起点的所述异源蛋白质的功能性表达盒的表达来表达。 本发明还包括转化的啮齿动物细胞系，用于在所述细胞系中产生异源蛋白质的方法和用于构建所述细胞系的试剂盒。
-
公开(公告)号：US20140227305A1
公开(公告)日：2014-08-14
申请号：US13818711
申请日：2011-08-25
IPC分类号： A61K39/245
CPC分类号： A61K39/245 , A61K39/12 , A61K2039/5258 , A61K2039/57 , A61K2039/575 , C07K14/005 , C07K2317/569 , C12N7/00 , C12N2710/16223 , C12N2710/16234 , C12N2710/16252 , C12N2710/16262 , C12N2710/16271
摘要： The present invention relates to a vaccine comprising a particle, said particle comprising (i) at least one Epstein-Barr virus (EBV) structural polypeptide, (ii) at least one EBV lytic polypeptide, (iii) membrane lipids, said particle being devoid of EBV DNA, wherein (a) the B-cell transformation capacity of one or more EBV polypeptides required for B-cell transformation as comprised in said particle is disabled while their immunogenicity is maintained; and/or (b) said particle is devoid of one or more EBV polypeptides required for B-cell transformation. Furthermore, the invention relates to a method for generating a particle, to a cell obtained in the method of the invention, a kit comprising the vaccine or the particle generated according to the method of the invention. Also, the invention relates to the use of the vaccine or the particle generated according to the method of the invention for generating CD8+ cells specific for an EBV antigen.
摘要翻译： 本发明涉及包含颗粒的疫苗，所述颗粒包含（i）至少一种爱泼斯坦 - 巴尔病毒（EBV）结构多肽，（ii）至少一种EBV溶解多肽，（iii）膜脂质，所述颗粒无缺陷 的EBV DNA，其中（a）包含在所述颗粒中的B细胞转化所需的一种或多种EBV多肽的B细胞转化能力在其免疫原性保持时被禁用; 和/或（b）所述颗粒缺乏B细胞转化所需的一种或多种EBV多肽。 此外，本发明涉及一种生成颗粒的方法，本发明方法获得的细胞包含根据本发明方法生成的疫苗或颗粒的试剂盒。 此外，本发明涉及根据本发明的方法产生的疫苗或颗粒用于产生对EBV抗原特异性的CD8 +细胞的用途。
-
3.发明公开公开(公告)号：US20230381300A1
公开(公告)日：2023-11-30
申请号：US18032764
申请日：2021-10-20
IPC分类号： A61K39/245 , C12N7/00
CPC分类号： A61K39/245 , C12N7/00 , C12N2710/16223 , C12N2710/16222 , C12N2710/16234 , C12N2710/16252 , A61K2039/5258
摘要： The invention provides a method for manufacturing a HEK293 cell line, which is capable of producing Epstein-Barr virus-like particles (EB-VLPs), as well as the HEK293 cell line obtainable by said method. The invention is further directed to a method for manufacturing EB-VLPs and a composition comprising EB-VLPs obtainable by said method for manufacturing EB-VLPs. Additionally, the invention provides a kit comprising EB-VLPs generated according to the method for manufacturing EB-VLPs. Further, the invention relates to a method for manufacturing a vaccine as well as the vaccine containing EB-VLPs obtainable by said method for manufacturing EB-VLPs.
-
公开(公告)号：US20160296618A1
公开(公告)日：2016-10-13
申请号：US15077477
申请日：2016-03-22
IPC分类号： A61K39/245 , C07K14/005 , C12N7/00
CPC分类号： A61K39/245 , A61K39/12 , A61K2039/5258 , A61K2039/57 , A61K2039/575 , C07K14/005 , C07K2317/569 , C12N7/00 , C12N2710/16223 , C12N2710/16234 , C12N2710/16252 , C12N2710/16262 , C12N2710/16271
摘要： The present invention relates to a vaccine comprising a particle, said particle comprising (i) at least one Epstein-Barr virus (EBV) structural polypeptide, (ii) at least one EBV lytic polypeptide, (iii) membrane lipids, said particle being devoid of EBV DNA, wherein (a) the B-cell transformation capacity of one or more EBV polypeptides required for B-cell transformation as comprised in said particle is disabled while their immunogenicity is maintained; and or (b) said particle is devoid of one or more EBV polypeptides required for B-cell transformation. Furthermore, the invention relates to a method for generating a particle, to a cell obtained in the method of the invention, a kit comprising the vaccine or the particle generated according in the method of the invention. Also, the invention relates to the use of the vaccine or the particle generated according to the method of the invention for generating CD8+ cells specific for an EBV antigen.
摘要翻译： 本发明涉及包含颗粒的疫苗，所述颗粒包含（i）至少一种爱泼斯坦 - 巴尔病毒（EBV）结构多肽，（ii）至少一种EBV溶解多肽，（iii）膜脂质，所述颗粒无缺陷 的EBV DNA，其中（a）包含在所述颗粒中的B细胞转化所需的一种或多种EBV多肽的B细胞转化能力在其免疫原性保持时被禁用; 和（b）所述颗粒没有B细胞转化所需的一种或多种EBV多肽。 此外，本发明涉及一种生成颗粒的方法，本发明方法获得的细胞，包含根据本发明方法生成的疫苗或颗粒的试剂盒。 此外，本发明涉及根据本发明的方法产生的疫苗或颗粒用于产生对EBV抗原特异性的CD8 +细胞的用途。
-
公开(公告)号：US07892790B2
公开(公告)日：2011-02-22
申请号：US12083535
申请日：2006-10-26
申请人： Ulrich Goepfert , Erhard Kopetzki
发明人： Ulrich Goepfert , Erhard Kopetzki
CPC分类号： C12N15/85 , C12N7/00 , C12N2710/16252 , C12N2800/108
摘要： A method is described for the expression of heterologous polypeptides in rodent cells. The method comprises the oriP/EBNA-1 episomal replication and maintenance system of the Epstein-Barr-Virus (EBV). With the stable integration of an EBNA-1-protein expression cassette under the control of a promoter into the genome of a rodent cell an EBNA-1-protein expression in the cells was obtained. The heterologous protein is expressed from an episome comprising an EBV origin of replication and a functional expression cassette of said heterologous protein. The invention further comprises transformed rodent cell lines, a method for the production of a heterologous protein in said cell lines and a kit for the construction of said cell lines.
摘要翻译： 描述了在啮齿动物细胞中表达异源多肽的方法。 该方法包括爱泼斯坦 - 巴尔病毒（EBV）的oriP / EBNA-1附加体复制和维持系统。 通过将启动子控制下的EBNA-1蛋白表达盒稳定整合到啮齿动物细胞的基因组中，获得细胞中的EBNA-1蛋白表达。 异源蛋白质由包含EBV复制起点的所述异源蛋白质的功能性表达盒的表达来表达。 本发明还包括转化的啮齿动物细胞系，用于在所述细胞系中产生异源蛋白质的方法和用于构建所述细胞系的试剂盒。
-
-
-
-
搜索结果
5 条记录 (耗时 0.019 秒)
