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公开(公告)号：US09260740B2
公开(公告)日：2016-02-16
申请号：US11922785
申请日：2005-06-23
Applicant: Rosemary Katherine Cameron Sharpin
Inventor： Rosemary Katherine Cameron Sharpin
CPC classification number: C12Q1/08 , C12M23/34 , C12M41/36 , C12Q1/04 , Y10T436/173076
Abstract: The contamination of foodstuffs, additives, cosmetics, pharmaceuticals and the like by undesirable micro-organisms is threat to public health. On occasion, the level of bacteria that conventional testing techniques are designed to look for is so low that the test result for the bacterium is “negative”. However, the bacterium although present in un-detectably small amounts may multiply under the right conditions and given the shelf life of the product, may be able to recover to an extent whereby public health is threatened. This problem is alleviated by the provision of micro-organism testing apparatus comprising (a) an incubator, and (b) a multi-compartment resealable container provided with or adapted to receive a growth medium in one compartment and having a growth medium additive in another compartment, the compartments being separated by a barrier whose removal or puncture will expose the medium to the additive.
Abstract translation: 不良微生物对食品，添加剂，化妆品，药品等的污染对公众健康构成威胁。 有时，传统测试技术设计的细菌水平很低，使得细菌的检测结果为“阴性”。 然而，细菌虽然以不可检测的少量存在，但可以在合适的条件下繁殖并给予产品的保存期限，可以恢复到公共卫生受到威胁的程度。 通过提供微生物测试装置来减轻这个问题，微生物测试装置包括（a）培养箱和（b）多室可重新密封的容器，其提供或适于在一个隔室中接收生长培养基并且在另一个隔室中具有生长培养基添加剂 隔间，隔室被隔离物隔开，其移除或穿刺将介质暴露于添加剂。
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公开(公告)号：US08642323B2
公开(公告)日：2014-02-04
申请号：US13351637
申请日：2012-01-17
Applicant: Rosemary Katherine Cameron Sharpin
Inventor： Rosemary Katherine Cameron Sharpin
CPC classification number: C12Q1/08 , C12M23/34 , C12M41/36 , C12Q1/04 , Y10T436/173076
Abstract: A multi-compartment resealable container for testing for the presence of micro-organisms is provided with or adapted to receive a sample in a first substantially rigid transparent compartment, a growth medium in a second compartment, and a sanitizer in a third compartment, the compartments being separated by foil seals, the second and third compartments have an associated plunger which when depressed causes serrated cutters to puncture the foil seal and allow the contents to be added to the liquid containing or comprising the sample.
Abstract translation: 提供用于测试微生物存在的多室可再密封容器，或适于在第一基本上刚性的透明隔室，第二隔室中的生长培养基和第三隔室中的消毒剂中接收样品， 被箔密封件隔开，第二和第三隔室具有相关联的柱塞，当压下时，锯齿形切割器刺穿箔密封并允许将内容物添加到含有或包含样品的液体中。
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Patent Agency Ranking
公开(公告)号：US20090111173A1
公开(公告)日：2009-04-30
申请号：US11922785
申请日：2005-06-23
Applicant: Rosemary Katherine Cameron Sharpin
Inventor： Rosemary Katherine Cameron Sharpin
IPC: C12M1/00
CPC classification number: C12Q1/08 , C12M23/34 , C12M41/36 , C12Q1/04 , Y10T436/173076
Abstract: A micro-organism testing apparatus comprising a multi-compartment resealable container provided with or adapted to receive a growth medium (97) in one compartment (83) and having a growth medium additive in another compartment (84, 85, 86), the compartments being separated by a barrier (95) whose removal or puncture will expose the medium to the additive. A barrier puncure mechanism, such as “saw tooth” shaped teeth can be used and further compartments and barrier puncture mechanisms can be used.
Abstract translation: 一种微生物测试装置，包括具有或适于在一个隔室（83）中容纳生长介质（97）并且在另一隔室（84,85,86）中具有生长培养基添加剂的多室可再密封容器，所述隔室 被隔离物（95）分开，其移除或穿刺将使介质暴露于添加剂。 可以使用诸如“锯齿”形齿之类的屏障打孔机构，并且可以使用进一步的隔室和屏障穿刺机构。
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公开(公告)号：US5716847A
公开(公告)日：1998-02-10
申请号：US539679
申请日：1995-10-05
Applicant: Maxine Helen Simmons , Rosemary Katherine Cameron Sharpin , Jeremy Gilbert Elliot Thompson
Inventor： Maxine Helen Simmons , Rosemary Katherine Cameron Sharpin , Jeremy Gilbert Elliot Thompson
CPC classification number: A01N1/02 , A01N1/0226
Abstract: Aqueous buffered physiological solutions suitable for use with embryos are disclosed based on a selection of zwitterionic buffers, allowing improved embryo solutions to be produced, giving a greater holding time than conventional phosphate buffers. An embryo flushing solution and an embryo holding solution based on these bufers are described. A preferred buffered embryo holding solution contains the following ingredients: NACl, MOPS (3-�N-morpholino! propane sulfonic acid!), KCl, CaCl.sub.2.2H.sub.2 O, MgCl.sub.2.6H.sub.2 O, NaHCO.sub.3, Kanamycin Sulphate, Glucose, Bovine Albumin, Na Lactate, NaOH, and tissue culture grade water.
Abstract translation: 基于两性离子缓冲液的选择公开了适用于胚胎的水性缓冲生理溶液，允许改进的胚胎溶液产生，给出比常规磷酸盐缓冲液更大的保持时间。 描述了基于这些絮状物的胚胎冲洗溶液和胚胎保持溶液。 优选的缓冲胚保持溶液含有以下成分：NAC1，MOPS（3- [N-吗啉代]丙磺酸]），KCl，CaCl 2·2H 2 O，MgCl 2·6H 2 O，NaHCO 3，卡那霉素硫酸盐，葡萄糖，牛白蛋白，乳酸钠 ，NaOH和组织培养级水。
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公开(公告)号：US20140255984A1
公开(公告)日：2014-09-11
申请号：US14128940
申请日：2012-06-21
Applicant: Rosemary Katherine Cameron Sharpin
Inventor： Rosemary Katherine Cameron Sharpin
CPC classification number: C12Q1/04 , C12Q1/10 , G01N1/00 , G01N21/51 , G01N2001/021 , G01N2001/022 , G01N2021/4726
Abstract: A method of tracking and predicting the spread of micro-organisms in an environment relies on the optical detection of the growth of micro-organisms within an analyte containing a growth medium for the micro-organism(s) to be detected. Standardisation of the containers and growth medium for each micro-organism allows rapid detection of the growth pattern of particular organisms and the implementation of a regime of remote self-reporting of results from portable incubator units (1201) in the field which include a memory (1206) to store the results, a GPS unit (1204) to provide location information and a transmitter (1203) to send the results to a central plotting organisation allowing rapid tracking and prediction of the spread of environmentally borne organisms.
Abstract translation: 跟踪和预测微生物在环境中的扩散的方法依赖于对含有待检测微生物的生长培养基的分析物中微生物生长的光学检测。 每个微生物的容器和生长培养基的标准化允许快速检测特定生物体的生长模式，以及实施远程自我报告结果的制度，该领域包括记录在内的便携式孵化器单元（1201） 1206）存储结果，提供位置信息的GPS单元（1204）和发送器（1203）以将结果发送到中央绘图组织，允许快速跟踪和预测环境传播的生物体的传播。
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公开(公告)号：US6051394A
公开(公告)日：2000-04-18
申请号：US254801
申请日：1999-03-11
Applicant: Maxine Helen Simmons , Rosemary Katherine Cameron Sharpin
CPC classification number: C12Q1/04 , Y10S435/975
Abstract: A method and kit for detecting micro-organisms in a mass or liquid or on a surface. The method is conducted by obtaining a sample and within a sterile chamber of limited volumetric capacity placing the sample in contact with a composition adapted to detect micro-organisms. The sterile chamber may contain a filter for isolating any micro-organisms present in the sample. Sterile sampling instruments, a delineating instrument and means for typing the micro-organisms may also be provided.
Abstract translation: PCT No.PCT / NZ97 / 00116 Sec。 371 1999年3月11日 102（e）1999年3月11日PCT 1997年9月12日PCT公布。 公开号WO98 / 11250 PCT 日期1998年3月19日用于检测质量或液体或表面上的微生物的方法和试剂盒。 该方法通过获得样品并在有限体积容量的无菌室内进行，将样品与适于检测微生物的组合物接触。 无菌室可以包含用于分离样品中存在的任何微生物的过滤器。 也可以提供无菌取样仪器，描绘仪器和用于分类微生物的装置。
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公开(公告)号：US5807684A
公开(公告)日：1998-09-15
申请号：US669420
申请日：1996-07-09
Applicant: Maxine Helen Simmons , Rosemary Katherine Cameron Sharpin
IPC: G01N33/04 , G01N33/53 , G01N33/537 , G01N33/68
CPC classification number: G01N33/6854
Abstract: A method of testing for the existence of mastitis in milk from cows either from an individual cow or from a herd of cows, by (a) obtaining a sample of milk to be tested, (b) determining whether immunoglobulin G2 (IgG.sub.2) is present in said sample at a level above or below a predetermined level which is typically about 0.05 mg IgG.sub.2 per milliliter of milk, by an ELISA comparison with immunoglobulin standards, and (c) classifying the individual cow or herd of cows as mastitis affected if said immunoglobulin is present in said sample at or above said predetermined level.
Abstract translation: PCT No.PCT / NZ94 / 00003 Sec。 371日期：1996年7月9日 102（e）日期1996年7月9日PCT 1994年1月24日PCT PCT。 WO95 / 20159 PCT出版物 日期1995年7月27日一种通过（a）获得要测试的牛奶样品，（b）确定免疫球蛋白G2是否确定是否测试来自单独的牛或来自母牛的牛的乳中存在乳腺炎的方法 （IgG2）以高于或低于预定水平的水平存在，通过ELISA与免疫球蛋白标准的比较，其通常为每毫升牛奶约0.05mg IgG2，和（c）将单独的牛或牛群分类为 如果所述免疫球蛋白在所述样品中存在或高于所述预定水平，则影响乳腺炎。
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