公开(公告)号：US20100248389A1

公开(公告)日：2010-09-30

申请号：US12679050

申请日：2008-09-22

申请人： Russell J. Stewart ,  Betsy J. Endrizzi ,  Gang Huang ,  Vladimir Hlady ,  Samuel Aaron Poff ,  Jennifer Schumaker-Parry

发明人： Russell J. Stewart ,  Betsy J. Endrizzi ,  Gang Huang ,  Vladimir Hlady ,  Samuel Aaron Poff ,  Jennifer Schumaker-Parry

IPC分类号： G01N33/53 ,  C25D11/00

CPC分类号： C08F2/58 ,  C09D5/4411 ,  C09D133/26

摘要： Described herein are methods for electrodepositing a variety of different polymers on metal substrates. The polymers are strongly adhered to the substrates. The substrates produced herein can be used in a number of different applications such as, for example, medical devices and biosensors. For example, the biosensors can be composed of one or more electrodes, where the electrodes have the same or different polymers electrochemically deposited on them. Finally, the methods described herein permit the evaluation of the electrodeposition process as well as monitor the ability of biomolecules to bind to the electrodeposited polymers.

摘要翻译： 这里描述的是在金属基底上电沉积各种不同的聚合物的方法。 聚合物牢固地附着在基材上。 本文生产的基材可用于许多不同的应用，例如医疗装置和生物传感器。 例如，生物传感器可以由一个或多个电极组成，其中电极具有电化学沉积在其上的相同或不同的聚合物。 最后，本文描述的方法允许电沉积过程的评估以及监测生物分子与电沉积聚合物结合的能力。

公开(公告)号：US06501549B1

公开(公告)日：2002-12-31

申请号：US09077694

申请日：1998-12-03

申请人： Joseph D. Andrade ,  Chung-Yih Wang ,  Vladimir Hlady ,  Philip M. Triolo ,  Robert J. Scheer

发明人： Joseph D. Andrade ,  Chung-Yih Wang ,  Vladimir Hlady ,  Philip M. Triolo ,  Robert J. Scheer

IPC分类号： G01B1100

CPC分类号： G01N21/763 ,  G01N21/6456 ,  G01N21/76

摘要： A method and associated apparatus for measuring chemical concentration in a liquid sample based on spatial separation and resolution of light is disclosed. The method is preferably applied to sensitive, quantitative, luminescence-based biosensors which reads the analyte concentration via spatial distribution of the emitted light. The detection of light is used to assess the spatial position, rather than the intensity or wavelength, of emitted light. A bioluminescent or chemiluminescent reaction requiring, for example, ATP, NADPH or NADH as a specific, and sensitive co-factor is used. ATP or NADH concentration is modulated, “tuned,” and/or regulated via, for example, an enzyme which consumes (“consumase”) ATP, NADPH, or NADH, thereby producing a spatial distribution of ATP or NADH and a spatial distribution in the emitted light. By appropriate control of the consumase or “synthase” activity and kinetics, a sensitive, specific, and easily readable luminescent pattern is produced, permitting detection.

摘要翻译： 公开了一种基于空间分离和光分辨率来测量液体样品中化学浓度的方法和相关装置。 该方法优选地应用于通过发射光的空间分布读取分析物浓度的灵敏的，定量的，基于发光的生物传感器。 光的检测用于评估发射光的空间位置，而不是强度或波长。 使用需要例如ATP，NADPH或NADH作为特异性和敏感辅因子的生物发光或化学发光反应。 ATP或NADH浓度通过例如消耗（“消耗”）ATP，NADPH或NADH的酶“调节”和/或调节，由此产生ATP或NADH的空间分布和空间分布 发出的光。 通过适当控制消耗或“合成酶”活性和动力学，产生敏感，特异且容易读取的发光图案，允许检测。

公开(公告)号：US5700953A

公开(公告)日：1997-12-23

申请号：US627948

申请日：1996-04-03

申请人： Vladimir Hlady ,  Andras Pungor ,  Eric W. Stroup

发明人： Vladimir Hlady ,  Andras Pungor ,  Eric W. Stroup

IPC分类号： G01B21/30 ,  G01N3/38 ,  G01Q10/04 ,  G01B5/28

CPC分类号： G01Q60/32 ,  B82Y35/00 ,  G01N3/38 ,  G01Q10/065 ,  G01N2203/0051 ,  Y10S977/852

摘要： The method for mapping a mechanical property of a surface of a sample with a scanning force microscope comprises the steps of (a) scanning a fine tip in contact with the surface of the sample within a predetermined scan area, the fine tip being supported on an end of a cantilever beam, (b) applying a loading force on the surface of the sample by the fine tip, (c) using a constant frequency sine wave to oscillate the cantilever beam relative to the surface of the sample, (d) measuring a detector response of the fine tip at the end of the cantilever beam, (e) determining an amplitude of the detector response and a change in phase angle of the detector response relative to the sine wave used to oscillate the cantilever beam, and (f) relating the amplitude and the change in phase angle to a property of the surface of the sample. A feedback system is utilized which regulates the total force imposed by the fine tip onto the surface of the sample so that the amplitude of the detector response is maintained constant. Because the force does not depend on the topography of the sample, the average cantilever deflection is directly related to the mechanical property of the surface of the sample and is not affected by any topographical feature present on the surface of the sample.

摘要翻译： 用扫描力显微镜绘制样品表面的力学性能的方法包括以下步骤：（a）在预定的扫描区域内扫描与样品表面接触的细小尖端，细尖端被支撑在 悬臂梁的端部，（b）通过细尖端在样品的表面上施加负载力，（c）使用恒定频率正弦波相对于样品的表面振荡悬臂梁，（d）测量 （e）确定检测器响应的幅度和相对于用于振荡悬臂梁的正弦波的检测器响应的相位角的变化的检测器响应，以及（f ）将相位角的振幅和变化与样品表面的性质相关联。 利用一种反馈系统，其调节细尖端施加到样品表面上的总力，使得检测器响应的幅度保持恒定。 由于力不依赖于样品的形貌，所以平均悬臂偏转与样品表面的机械性能直接相关，并且不受样品表面上存在的任何形貌特征的影响。