-
公开(公告)号：US11248217B2
公开(公告)日：2022-02-15
申请号：US16545786
申请日：2019-08-20
摘要： The present invention relates to engineered carbohydrate-active enzyme constructs that are useful for glycan polymers synthesis. The construct comprises a CD domain from a GH, wherein the domain is conjugated to CBM3a via a peptidic linker. The invention also relates to a method of improving glycan polymer synthesis by using engineered carbohydrate active enzymes comprising a CD domain and CBM3a.
-
公开(公告)号：US20230279464A1
公开(公告)日：2023-09-07
申请号：US18010109
申请日：2021-06-28
IPC分类号： C12Q1/40 , C12Q1/6897 , C12N15/70
CPC分类号： C12Q1/40 , C12Q1/6897 , C12Y302/01018 , C12N15/70 , C12N2800/101
摘要： The present disclosure provides, in one aspect, an azide-inducible system for controlled gene expression in E. coli. In another aspect, the present disclosure provides a high throughput screening method for the identification of new glycosynthases (e.g., mutant glycosyl hydrolases) with enhanced activity and unique substrate specificity.
-
公开(公告)号：US20200056215A1
公开(公告)日：2020-02-20
申请号：US16545786
申请日：2019-08-20
摘要： The present invention relates to engineered carbohydrate-active enzyme constructs that are useful for glycan polymers synthesis. The construct comprises a CD domain from a GH, wherein the domain is conjugated to CBM3a via a peptidic linker. The invention also relates to a method of improving glycan polymer synthesis by using engineered carbohydrate active enzymes comprising a CD domain and CBM3a.
-
4.发明申请公开(公告)号：US20210024974A1
公开(公告)日：2021-01-28
申请号：US16935286
申请日：2020-07-22
摘要： The present disclosure relates, in one aspect, to the discovery of a high throughput screening (HTS) method to rapidly screen for GH/GS variants that are generated using directed evolution techniques and that can significantly enhance glycosynthase catalytic activity or product specificity.
-
-
-
搜索结果
4 条记录 (耗时 0.015 秒)
