公开(公告)号：US06823508B1

公开(公告)日：2004-11-23

申请号：US09560676

申请日：2000-04-27

申请人： Ryan Burkhardt ,  Tom G. Yaryan ,  Seetharaman Harikrishnnan ,  Donald J. McNamara ,  David J. D'Souza ,  Nicholas R. Legget ,  David Scott Johnson ,  Seung-Yup Chai

发明人： Ryan Burkhardt ,  Tom G. Yaryan ,  Seetharaman Harikrishnnan ,  Donald J. McNamara ,  David J. D'Souza ,  Nicholas R. Legget ,  David Scott Johnson ,  Seung-Yup Chai

IPC分类号： G06F9445

CPC分类号： G06F8/61 ,  G06F9/44505

摘要： Software programs, such as an operating system or other application programs, are automatically customized to a specific user(s) based on data corresponding to the specific user(s) that is maintained in a user information store. In one embodiment, the information store is a unified store that is accessible by multiple programs including the operating system. Thus, new information or information changes can be made available to multiple programs by the user adding (or changing) the information only once. In another embodiment, the operating system image to be installed on a computer is pre-populated with user-specific information at the factory. The user-specific information can be integrated into the operating system at the factory or alternatively upon an initial boot of the computer by the user.

摘要翻译： 基于与在用户信息存储器中维护的特定用户相对应的数据，诸如操作系统或其他应用程序的软件程序被自动地定制到特定用户。 在一个实施例中，信息存储是可由包括操作系统的多个程序访问的统一存储。 因此，用户只能添加（或更改）信息一次，可以向多个程序提供新的信息或信息更改。 在另一个实施例中，要安装在计算机上的操作系统映像在工厂预填充用户特定的信息。 用户特定的信息可以在出厂时被整合到操作系统中，或者在使用者初始引导计算机时。

公开(公告)号：US07596785B2

公开(公告)日：2009-09-29

申请号：US10961800

申请日：2004-10-08

申请人： Ryan Burkhardt ,  Tom G. Yaryan ,  Seetharaman Harikrishnnan ,  Donald J. McNamara ,  David J. D'Souza ,  Nicholas R. Legget ,  David Scott Johnson ,  Seung-Yup Chai

发明人： Ryan Burkhardt ,  Tom G. Yaryan ,  Seetharaman Harikrishnnan ,  Donald J. McNamara ,  David J. D'Souza ,  Nicholas R. Legget ,  David Scott Johnson ,  Seung-Yup Chai

IPC分类号： G06F9/445

CPC分类号： G06F8/61 ,  G06F9/44505

摘要： Software programs, such as an operating system or other application programs, are automatically customized to a specific user(s) based on data corresponding to the specific user(s) that is maintained in a user information store. In one embodiment, the information store is a unified store that is accessible by multiple programs including the operating system. Thus, new information or information changes can be made available to multiple programs by the user adding (or changing) the information only once. In another embodiment, the operating system image to be installed on a computer is pre-populated with user-specific information at the factory. The user-specific information can be integrated into the operating system at the factory or alternatively upon an initial boot of the computer by the user.

摘要翻译： 基于与在用户信息存储器中维护的特定用户相对应的数据，诸如操作系统或其他应用程序的软件程序被自动地定制到特定用户。 在一个实施例中，信息存储是可由包括操作系统的多个程序访问的统一存储。 因此，用户只能添加（或更改）信息一次，可以向多个程序提供新的信息或信息更改。 在另一个实施例中，要安装在计算机上的操作系统映像在工厂预填充用户特定的信息。 用户特定的信息可以在出厂时被整合到操作系统中，或者在使用者初始引导计算机时。

公开(公告)号：US20100017798A1

公开(公告)日：2010-01-21

申请号：US12566583

申请日：2009-09-24

申请人： Ryan Burkhardt ,  Tom G. Yaryan ,  Seetharaman Harikrishnan ,  Donald J. McNamara ,  David J. D'Souza ,  Nicholas R. Legget ,  David Scott Johnson ,  Seung-Yup Chai

发明人： Ryan Burkhardt ,  Tom G. Yaryan ,  Seetharaman Harikrishnan ,  Donald J. McNamara ,  David J. D'Souza ,  Nicholas R. Legget ,  David Scott Johnson ,  Seung-Yup Chai

IPC分类号： G06F9/445

CPC分类号： G06F8/61 ,  G06F9/44505

摘要： Software programs, such as an operating system or other application programs, are automatically customized to a specific user(s) based on data corresponding to the specific user(s) that is maintained in a user information store. In one embodiment, the information store is a unified store that is accessible by multiple programs including the operating system. Thus, new information or information changes can be made available to multiple programs by the user adding (or changing) the information only once. In another embodiment, the operating system image to be installed on a computer is pre-populated with user-specific information at the factory. The user-specific information can be integrated into the operating system at the factory or alternatively upon an initial boot of the computer by the user.

摘要翻译： 基于与在用户信息存储器中维护的特定用户相对应的数据，诸如操作系统或其他应用程序的软件程序被自动地定制到特定用户。 在一个实施例中，信息存储是可以由包括操作系统的多个程序访问的统一存储。 因此，用户只能添加（或更改）信息一次，可以向多个程序提供新的信息或信息更改。 在另一个实施例中，要安装在计算机上的操作系统映像在工厂预填充用户特定的信息。 用户特定的信息可以在出厂时被整合到操作系统中，或者在使用者初始引导计算机时。

公开(公告)号：US08515679B2

公开(公告)日：2013-08-20

申请号：US12076348

申请日：2008-03-17

申请人： Matthew Rabinowitz ,  Milena Banjevic ,  Zachary Demko ,  David Scott Johnson ,  Dusan Kijacic ,  Dimitri Petrov ,  Josh Sweetkind-Singer ,  Jing Xu

发明人： Matthew Rabinowitz ,  Milena Banjevic ,  Zachary Demko ,  David Scott Johnson ,  Dusan Kijacic ,  Dimitri Petrov ,  Josh Sweetkind-Singer ,  Jing Xu

IPC分类号： G01N33/48 ,  G01N31/00 ,  G06G7/48 ,  G06G7/58

CPC分类号： G06F19/16 ,  C12Q1/6855 ,  C12Q1/6874 ,  G06F19/18 ,  G06F19/24 ,  G06Q50/22

摘要： Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Genetic material from the target individual is acquired, amplified and the genetic data is measured using known methods. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment of the invention, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment of the invention, the chromosome copy number can be determined from the measured genetic data of a single or small number of cells, with or without genetic information from one or both parents. In another embodiment of the invention, these determinations are made for the purpose of embryo selection in the context of in-vitro fertilization. In another embodiment of the invention, the genetic data can be reconstructed for the purposes of making phenotypic predictions.

摘要翻译： 本文公开了一种系统和方法，用于增加测量的遗传数据的保真度，进行等位基因调用，并确定在一组或一组细胞中或从片段DNA中确定非整倍体的状态，其中有限数量的遗传数据 是可用的 获取，扩增来自目标个体的遗传物质，并使用已知方法测量遗传数据。 使用目标基因组与遗传相关个体的基因组之间的预期相似性来重建差或不正确测量的碱基对，缺失的等位基因和缺失区域。 根据本发明的一个实施方案，使用来自一个或两个亲本的二倍体细胞的较大样本的更完整的遗传数据，在多个基因座重建来自胚胎细胞的不完全遗传数据，具有或不具有来自一个的单倍体遗传数据 或双亲。 在本发明的另一个实施方案中，染色体拷贝数可以从单个或少数细胞的测量的遗传数据确定，具有或不具有来自一个或两个亲本的遗传信息。 在本发明的另一个实施方案中，这些确定是为了在体外受精的背景下进行胚胎选择的目的。 在本发明的另一个实施方案中，为了进行表型预测的目的，可以重建遗传数据。

公开(公告)号：US20140057799A1

公开(公告)日：2014-02-27

申请号：US13993047

申请日：2011-12-16

申请人： David Scott Johnson ,  Everett Hurteau Meyer

发明人： David Scott Johnson ,  Everett Hurteau Meyer

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C07K16/00 ,  C07K2317/622 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1075 ,  C12Q1/6837 ,  C12Q1/6846 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/6888 ,  C12Q2600/156 ,  C40B50/06 ,  C12Q2525/161 ,  C12Q2525/307 ,  C12Q2563/159

摘要： Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

摘要翻译： 提供了用于大规模并行遗传分析乳液液滴或反应容器中单细胞的方法和系统。 感兴趣的遗传基因座使用一组探针靶向单个细胞，通过分子连锁和扩增技术形成融合复合体。 提供了用于在至少10,000个细胞群体中的单个细胞中的融合复合物的高通量，大规模并行分析的方法。 还提供了使用条形码序列追踪遗传信息回到细胞的方法。

公开(公告)号：US08532930B2

公开(公告)日：2013-09-10

申请号：US11603406

申请日：2006-11-22

申请人： Matthew Rabinowitz ,  Milena Banjevic ,  Zachary Paul Demko ,  David Scott Johnson

发明人： Matthew Rabinowitz ,  Milena Banjevic ,  Zachary Paul Demko ,  David Scott Johnson

IPC分类号： G01N33/48 ,  G01N31/00 ,  G06G7/48 ,  G06G7/58

CPC分类号： G06F19/20 ,  G06F19/18

摘要： A system and method for determining the genetic data for one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Genetic data for the target individual is acquired and amplified using known methods, and poorly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related subjects. In accordance with one embodiment of the invention, incomplete genetic data from an embryonic cell is reconstructed using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without genetic data from haploid cells from one or both parents, and/or genetic data taken from other related individuals. In accordance with another embodiment of the invention, incomplete genetic data from a fetus is acquired from fetal cells, or cell-free fetal DNA isolated from the mother's blood, and the incomplete genetic data is reconstructed using the more complete genetic data from a larger sample diploid cells from one or both parents, with or without genetic data from haploid cells from one or both parents, and/or genetic data taken from other related individuals. In one embodiment, the genetic data can be reconstructed for the purposes of making phenotypic predictions. In another embodiment, the genetic data can be used to detect for aneuploides and uniparental disomy.

摘要翻译： 用于确定有限数量的遗传数据可用的一个或一小组细胞或来自片段DNA的遗传数据的系统和方法。 使用已知方法获取和扩增目标个体的遗传数据，并且使用目标基因组与基因相关受试者的基因组之间的预期相似性重建差测量的碱基对，缺失的等位基因和缺失区域。 根据本发明的一个实施方案，使用来自一个或两个亲本的二倍体细胞的较大样本的更完整的遗传数据重建来自胚胎细胞的不完整遗传数据，具有或不具有来自一个或两个亲本的单倍体细胞的遗传数据 ，和/或从其他相关个体获取的遗传数据。 根据本发明的另一个实施方案，从胎儿细胞或从母体血液中分离的无细胞胎儿DNA获得来自胎儿的不完整遗传数据，并且使用来自较大样本的更完整的遗传数据重建不完全遗传数据 来自一个或两个父母的二倍体细胞，具有或不具有来自一个或两个亲本的单倍体细胞的遗传数据和/或从其他相关个体获取的遗传数据。 在一个实施方案中，为了进行表型预测的目的，可以重建遗传数据。 在另一个实施方案中，遗传数据可用于检测非整倍体和单倍体二体解剖。

公开(公告)号：US06735691B1

公开(公告)日：2004-05-11

申请号：US09492899

申请日：2000-01-27

申请人： Stephen P. Capps ,  David A. Feinleib ,  Jacob D. Swed ,  David Scott Johnson

发明人： Stephen P. Capps ,  David A. Feinleib ,  Jacob D. Swed ,  David Scott Johnson

IPC分类号： G06F900

CPC分类号： G06F9/44505

摘要： A system and method for configuring a computer involves first compiling configuration information from a source computer and populating a migration file with the configuration information. The migration file is kept at a server. Subsequently, upon access to the server, the configuration information from the migration file is retrieved and used to configure a destination computer.

摘要翻译： 用于配置计算机的系统和方法包括首先从源计算机编译配置信息并使用配置信息填充迁移文件。 迁移文件保存在服务器上。 随后，在访问服务器时，检索来自迁移文件的配置信息，并用于配置目标计算机。

公开(公告)号：US20080243398A1

公开(公告)日：2008-10-02

申请号：US12076348

申请日：2008-03-17

申请人： Matthew Rabinowitz ,  Milena Banjevic ,  Zachary Demko ,  David Scott Johnson ,  Dusan Kijacic ,  Dimitri Petrov ,  Josh Sweetkind-Singer ,  Jing Xu

发明人： Matthew Rabinowitz ,  Milena Banjevic ,  Zachary Demko ,  David Scott Johnson ,  Dusan Kijacic ,  Dimitri Petrov ,  Josh Sweetkind-Singer ,  Jing Xu

IPC分类号： G01N33/48

CPC分类号： G06F19/16 ,  C12Q1/6855 ,  C12Q1/6874 ,  G06F19/18 ,  G06F19/24 ,  G06Q50/22

摘要： Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Genetic material from the target individual is acquired, amplified and the genetic data is measured using known methods. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment of the invention, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment of the invention, the chromosome copy number can be determined from the measured genetic data of a single or small number of cells, with or without genetic information from one or both parents. In another embodiment of the invention, these determinations are made for the purpose of embryo selection in the context of in-vitro fertilization. In another embodiment of the invention, the genetic data can be reconstructed for the purposes of making phenotypic predictions.

摘要翻译： 本文公开了一种系统和方法，用于增加测量的遗传数据的保真度，进行等位基因调用，并确定在一组或一组细胞中或从片段DNA中确定非整倍体的状态，其中有限数量的遗传数据 是可用的 获取，扩增来自目标个体的遗传物质，并使用已知方法测量遗传数据。 使用目标基因组与遗传相关个体的基因组之间的预期相似性来重建差或不正确测量的碱基对，缺失的等位基因和缺失区域。 根据本发明的一个实施方案，使用来自一个或两个亲本的二倍体细胞的较大样本的更完整的遗传数据，在多个基因座重建来自胚胎细胞的不完全遗传数据，具有或不具有来自一个的单倍体遗传数据 或双亲。 在本发明的另一个实施方案中，染色体拷贝数可以从单个或少数细胞的测量的遗传数据确定，具有或不具有来自一个或两个亲本的遗传信息。 在本发明的另一个实施方案中，这些确定是为了在体外受精的背景下进行胚胎选择的目的。 在本发明的另一个实施方案中，为了进行表型预测的目的，可以重建遗传数据。