公开(公告)号：US11572578B2

公开(公告)日：2023-02-07

申请号：US17263851

申请日：2019-07-31

Applicant: SARAYA CO., LTD.

Inventor： Yoriko Kato ,  Yuka Oda ,  Emiko Kawamukai ,  Yoshihiko Hirata

IPC: C12Q1/04

Abstract: The present invention relates to a reagent kit for detecting biofilm in test tissue, the reagent kit comprising: (a) a pretreatment liquid comprising at least one surfactant selected, from the group consisting of nonionic surfactants, amphoteric surfactants, and cationic surfactants, (b) a staining liquid comprising a dye, and (c) a decolorizing liquid comprising at least one surfactant selected from the group consisting of nonionic surfactants, amphoteric surfactants, and cationic surfactants; wherein after a membrane is brought into contact with the test tissue and released from contact, the pretreatment liquid (a), the staining liquid (b), and the decolorizing liquid (c) are brought into contact with the contact surface of the membrane in this order.

公开(公告)号：US20210041448A1

公开(公告)日：2021-02-11

申请号：US17048278

申请日：2019-04-02

Applicant: The University of Tokyo ,  Saraya Co., Ltd.

Inventor： Takeo Minematsu ,  Hiromi Sanada ,  Nao Tamai ,  Yoshihiko Hirata ,  Yuka Oda

IPC: G01N33/68 ,  G01N1/30 ,  G01N33/573 ,  A61B10/00

Abstract: A problem to be solved by the present invention is to provide a method of assisting diagnosis of the health condition of a subject by correcting variation in an amount of a collected disease marker, which is caused by variation in the skin barrier function of the subject, and acquiring information reflecting the health condition of the subject accurately, and to solve the problem, the present invention provides a method of assisting diagnosis of a disease in a subject using a disease marker and a reference marker, the method including: applying, to a skin surface of the subject, a sheet capable of generating an attractive force due to electrostatic interaction between the sheet and each of the disease and reference markers; detaching the sheet from the skin surface; measuring the amount of each of the disease and reference markers that are attached to the sheet; and acquiring information on the disease in the subject based on a value obtained by correcting the measured amount of the disease marker with the measured amount of the reference marker, wherein the reference marker is annexin A2.