公开(公告)号：US20200207804A1

公开(公告)日：2020-07-02

申请号：US16500812

申请日：2018-04-03

Applicant: SCARAB GENOMICS, LLC

Inventor： Frederick R. BLATTNER ,  David FRISCH ,  Charles LANDRY ,  John BRANDON

IPC: C07K1/16 ,  C07K14/34 ,  C07K1/18 ,  B01D15/38 ,  B01D15/36 ,  B01D15/32 ,  B01D15/18

Abstract: The present invention relates to the use of redox agents for purification of the CRM 197 variant of diphtheria toxin. The invention further relates to multi-step purification of CRM 197 from bacterial fermentates.

公开(公告)号：US20170073379A1

公开(公告)日：2017-03-16

申请号：US15122891

申请日：2015-03-02

Applicant: SCARAB GENOMICS, LLC

Inventor： Cristopher R. BLATTNER ,  David FRISCH ,  Robert E. NOVY ,  Terrance M. HENKER ,  Eric A. STEFFEN ,  Frederick R. BLATTNER ,  Hyunsic CHOI ,  Gyorgy Posfai ,  Charles F. LANDRY

IPC: C07K14/34 ,  C12N1/20 ,  C12N1/08

CPC classification number: C07K14/34 ,  C12N1/08 ,  C12N1/20

Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Abstract translation: 提供了包含编码重组CRM197蛋白的表达载体及其在生产CRM 197中的应用的经重修的基因组或天然K12菌株的大肠杆菌细菌。 CRM 197蛋白可以融合到将表达的CRM197蛋白质引导到大肠杆菌宿主的周质的信号序列上。