公开(公告)号：US11407793B2

公开(公告)日：2022-08-09

申请号：US17262960

申请日：2019-11-20

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda Song ,  Junhuan Yang ,  Wu Yang ,  Shaoqi Li

IPC: C07K14/37 ,  C12N15/80

Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.

公开(公告)号：US11414650B2

公开(公告)日：2022-08-16

申请号：US17120163

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda Song ,  Md. Ahsanul Kabir Khan ,  Junhuan Yang ,  Huaiyuan Zhang ,  Wu Yang ,  Qing Liu

IPC: C12N9/02 ,  C12P7/64 ,  C12P7/6463 ,  C12N1/14 ,  C12N15/80

Abstract: The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-γ-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, γ-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-γ-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids. Under optimized fermentation conditions and in the presence of precursor fatty acid, the DGLA content reaches 7.6%. The new recombinant strain was deposited in China General Microbiological Culture Collection Center on Jun. 20, 2018, with the address of No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The accession number given to the biological material by the collection center is CGMCC No. 15887, and the suggested taxonomic denomination is Mucor circinelloides-GLELO.

公开(公告)号：US11345937B2

公开(公告)日：2022-05-31

申请号：US17120160

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda Song ,  Md. Ahsanul Kabir Khan ,  Junhuan Yang ,  Yao Zhang ,  Wu Yang ,  Shaoqi Li

IPC: C12P7/62 ,  C12P7/6427 ,  C12N9/02

Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid (SDA) and fermentation technology. Δ15 Desaturase gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and Δ15 Desaturase gene is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-Δ15, finally, the expression of the Δ15 Desaturase gene in Mucor circinelloides is realized. The recombinant new strain is accession number CGMCC No. 15888, and the classification name is Mucor circinelloides-D15D.