公开(公告)号：US11345937B2

公开(公告)日：2022-05-31

申请号：US17120160

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda Song ,  Md. Ahsanul Kabir Khan ,  Junhuan Yang ,  Yao Zhang ,  Wu Yang ,  Shaoqi Li

IPC: C12P7/62 ,  C12P7/6427 ,  C12N9/02

Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid (SDA) and fermentation technology. Δ15 Desaturase gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and Δ15 Desaturase gene is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-Δ15, finally, the expression of the Δ15 Desaturase gene in Mucor circinelloides is realized. The recombinant new strain is accession number CGMCC No. 15888, and the classification name is Mucor circinelloides-D15D.