公开(公告)号：US20160185742A1

公开(公告)日：2016-06-30

申请号：US14904695

申请日：2014-04-21

申请人： SHANGHAI JIAO TONG UNIVERSITY

发明人： Minjuan Xu ,  Jun Xu ,  Zhigang Zhang ,  Yahui Wang

IPC分类号： C07D311/58 ,  C12P17/06

CPC分类号： C07D311/58 ,  C12P17/06 ,  C12R1/465

摘要： The present invention opens to the public a method to prepare a benzopyran compound and its use for treating pulmonary fibrosis. A benzopyran compound has a structure (I): in which: R1 represents hydrogen, C1-C4 alkyl, or various remaining amino acid moieties after removal of an amino group; R2 represents hydrogen, C1-C4 alkyl, or various remaining amino acid moieties after removal of an amino group; R3 represents hydrogen, or C1-C4 alkyl; and n is any integer of 1-4, wherein the benzopyran compound is derived from a broth of Streptomyces xiamenensis CGMCC No. 5675 by extraction, separation and purification. The derivatives of xiamenmycin made from the present invention have a higher bioactivity to suppress the proliferation of normal human lung fibroblast, and medicinal products containing the same are useful in the treatment of pulmonary fibrosis.

摘要翻译： 本发明向公众公开了制备苯并吡喃化合物的方法及其用于治疗肺纤维化的用途。 苯并吡喃化合物具有结构（I）：其中：R 1表示氢，C 1 -C 4烷基或除去氨基后的各种残留氨基酸部分; 除去氨基后，R 2表示氢，C 1 -C 4烷基或各种残留的氨基酸部分; R3代表氢或C1-C4烷基; n为1-4的任意整数，其中苯并吡喃化合物是通过提取，分离和纯化得自厦门蓝链霉菌CGMCC号5675的肉汤。 由本发明制备的厦门霉素衍生物具有较高的生物活性以抑制正常人肺成纤维细胞的增殖，含有该成纤维细胞的药物可用于治疗肺纤维化。

公开(公告)号：US09944962B2

公开(公告)日：2018-04-17

申请号：US14902342

申请日：2014-04-21

申请人： SHANGHAI JIAO TONG UNIVERSITY

发明人： Jun Xu ,  Yong Yang ,  Ling Fu ,  Minjuan Xu ,  Zhongyuan You

IPC分类号： C12P17/06 ,  C12N15/52 ,  C12R1/465

CPC分类号： C12P17/06 ,  C12N15/52 ,  C12R1/465

摘要： The present invention involves the application of a gene cluster, and bacterial strains that used in the biosynthesis of Xiamenmycin. The nucleotide sequence of the gene cluster is showed as SEQ ID NO.1, with the whole length of 5189 base pairs. The present invention involves Streptomyces xiamenensis CGMCC No. 5670 and its mutant strain, and the method that how the mentioned bacterial strain produces Xiamenmycin. The present invention provides the strain sources by using Streptomyces xiamenensis, its mutant strains or genetically engineered microbial strains carrying the above-mentioned Xiamenmycin biosynthesis gene cluster for cultivation and produce benzopyran compound Xiamenmycin through biosynthesis. The mutant strains can be used in industrial production.

公开(公告)号：US20160222422A1

公开(公告)日：2016-08-04

申请号：US14902342

申请日：2014-04-21

申请人： SHANGHAI JIAO TONG UNIVERSITY

发明人： Jun Xu ,  Yong Yang ,  Ling FU ,  Minjuan Xu ,  Zhongyuan You

IPC分类号： C12P17/06 ,  C12N15/52

CPC分类号： C12P17/06 ,  C12N15/52 ,  C12R1/465

摘要： The present invention involves the application of a gene cluster, and bacterial strains that used in the biosynthesis of Xiamenmycin. The nucleotide sequence of the gene cluster is showed as SEQ ID NO.1, with the whole length of 5189 base pairs. The present invention involves Streptomyces xiamenensis CGMCC No. 5670 and its mutant strain, and the method that how the mentioned bacterial strain produces Xiamenmycin. The present invention provides the strain sources by using Streptomyces xiamenensis, its mutant strains or genetically engineered microbial strains carring the above-mentioned Xiamenmycin biosynthesis gene cluster for cultivation and produce benzopyran compound Xiamenmycin through biosynthesis. The mutant strains can be used in industrial production.

摘要翻译： 本发明涉及应用基因簇和用于厦门霉素生物合成的细菌菌株。 基因簇的核苷酸序列显示为SEQ ID NO.1，全长为5189个碱基对。 本发明涉及厦门蓝链霉菌CGMCC号5670及其突变菌株，以及所述菌株如何产生厦门霉素的方法。 本发明通过使用厦门绿链霉菌（Streptomyces xiamenensis），其突变菌株或携带上述厦门霉素生物合成基因簇的遗传工程微生物菌株进行培养并通过生物合成产生苯并吡喃化合物厦门霉素来提供菌株来源。 突变菌株可用于工业生产。

公开(公告)号：US10017489B2

公开(公告)日：2018-07-10

申请号：US14904695

申请日：2014-04-21

申请人： SHANGHAI JIAO TONG UNIVERSITY

发明人： Minjuan Xu ,  Jun Xu ,  Zhigang Zhang ,  Yahui Wang

IPC分类号： C07D311/00 ,  C07D311/58 ,  C12P17/06 ,  C12R1/465

CPC分类号： C07D311/58 ,  C12P17/06 ,  C12R1/465

摘要： The present invention opens to the public a method to prepare a benzopyran compound and its use for treating pulmonary fibrosis. A benzopyran compound has a structure (I): in which: R1 represents hydrogen, C1-C4 alkyl, or various remaining amino acid moieties after removal of an amino group; R2 represents hydrogen, C1-C4 alkyl, or various remaining amino acid moieties after removal of an amino group; R3 represents hydrogen, or C1-C4 alkyl; and n is any integer of 1-4, wherein the benzopyran compound is derived from a broth of Streptomyces xiamenensis CGMCC No. 5675 by extraction, separation and purification. The derivatives of xiamenmycin made from the present invention have a higher bioactivity to suppress the proliferation of normal human lung fibroblast, and medicinal products containing the same are useful in the treatment of pulmonary fibrosis.