公开(公告)号：US12163123B2

公开(公告)日：2024-12-10

申请号：US17292941

申请日：2018-11-22

Applicant: SHIMADZU CORPORATION

Inventor： Seiya Fujiwara ,  Masaki Kanai ,  Hiroyuki Jikuya ,  Tetsuo Ohashi ,  Masamitsu Shikata ,  Ayaka Minamimoto

IPC: C12N15/10 ,  B01J19/08 ,  B03C1/005 ,  B03C1/28

Abstract: A purpose of the present invention is to suppress reduction in recovery rate of a target component due to a gel adhering to an inner wall surface of a treatment liquid layer. After passing magnetic particles through a gel layer, when moving the magnetic particles in the treatment liquid layer adjacent to the gel layer, the magnetic force source is automatically operated in a longitudinal direction of a tubular container so that the magnetic particles do not enter a range of a certain distance from the gel layer through which the magnetic particles have passed.

公开(公告)号：US12263489B2

公开(公告)日：2025-04-01

申请号：US17840568

申请日：2022-06-14

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu Shikata ,  Ayaka Minamimoto ,  Akira Muramatsu ,  Masaki Kanai ,  Seiya Fujiwara ,  Tetsuo Ohashi ,  Hiroyuki Jikuya

IPC: B03C1/12 ,  B01D15/38 ,  B01L3/00 ,  B01L9/06 ,  B03C1/01 ,  B03C1/03 ,  B03C1/033 ,  B03C1/28 ,  B03C1/30 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6865

Abstract: A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

公开(公告)号：US10684279B2

公开(公告)日：2020-06-16

申请号：US15891509

申请日：2018-02-08

Applicant: SHIMADZU CORPORATION

Inventor： Masaki Kanai ,  Hiroyuki Jikuya ,  Tetsuo Ohashi ,  Seiya Fujiwara

IPC: G01N33/543 ,  G01N1/34 ,  B03C1/00 ,  G01N1/40

Abstract: To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.

公开(公告)号：US11883831B2

公开(公告)日：2024-01-30

申请号：US16603235

申请日：2017-04-06

Applicant: Shimadzu Corporation

Inventor： Masaki Kanai ,  Hiroyuki Jikuya ,  Tetsuo Ohashi ,  Seiya Fujiwara

IPC: B03C1/32 ,  C12Q1/68 ,  G01N35/08 ,  B01L3/00 ,  C12Q1/6806

CPC classification number: B03C1/32 ,  B01L3/502 ,  C12Q1/6806 ,  G01N35/08 ,  B01L2400/043

Abstract: A magnetic particle operation device comprising a tubular container having a longitudinal shape and including a sample introduction space in which a sample including a target substance and magnetic particles for immobilizing the target substance thereon are received, and a sample movement space in which a gel-like medium layer and a liquid layer are alternately arranged in its longitudinal direction. The magnetic particle operation device further comprising a first magnet provided outside the tubular container to face the sample introduction space, the first magnet configured to hold the magnetic particles in the sample introduction space in the container when the device is not used; and a second magnet configured to move the magnetic particles with the target substance being immobilized thereon, from the sample introduction space to the sample movement space, passing through the gel-like medium layer and the liquid layer in the longitudinal direction, when the device is used.