公开(公告)号：US20210139567A1

公开(公告)日：2021-05-13

申请号：US17096203

申请日：2020-11-12

Applicant: SHIMADZU CORPORATION ,  OSAKA INSTITUTE OF PUBLIC HEALTH

Inventor： Koichi KOJIMA ,  Kanae TERAMOTO ,  Shinichi IWAMOTO ,  Yuki WAKABAYASHI ,  Junko SAKATA

IPC: C07K16/12 ,  G01N33/68 ,  G01N30/72

Abstract: A method for detecting verotoxin includes: providing a sample and a molecule that binds to verotoxin; performing an operation for purifying verotoxin in the sample by using binding of the molecule and the verotoxin; and subjecting the sample obtained by the operation to a first mass spectrometry.

公开(公告)号：US20240192222A1

公开(公告)日：2024-06-13

申请号：US18078171

申请日：2022-12-09

Applicant: SHIMADZU CORPORATION

Inventor： Kanae TERAMOTO

IPC: G01N33/68 ,  H01J49/00 ,  H01J49/16

CPC classification number: G01N33/6851 ,  H01J49/0036 ,  H01J49/164

Abstract: Provided is a ribosomal protein judgement method, comprising: an attribution step that involves comparing observed m/z values indicated by peaks on a mass spectrum detected by mass spectrometry of a sample containing ribosomal proteins, with first calculated m/z values based on a database, and attributing at least some of the peaks thus detected to ribosomal proteins; and a presumption step that involves presuming a protein corresponding to a peak with a relative intensity from 50 to 150% relative to an approximate curve or an approximate straight line plotted with relative intensities of the peaks attributed to ribosomal proteins, to be a ribosomal protein.

公开(公告)号：US20240290602A1

公开(公告)日：2024-08-29

申请号：US18574591

申请日：2022-06-22

Applicant: SHIMADZU CORPORATION

Inventor： Kanae TERAMOTO

IPC: H01J49/16

CPC classification number: H01J49/164

Abstract: A microorganism mass spectrometry method includes: forming, on a sample plate, a mixed crystal of a matrix material and a microorganism sample which is a sample including a test microorganism or a component derived from the test microorganism; performing, in a mass spectrometer for performing analysis through matrix-assisted laser desorption/ionization mass spectrometry, a first laser irradiation in which a predetermined micro region on the mixed crystal is irradiated with a laser beam for a predetermined number of times; performing, in the mass spectrometer, a second laser irradiation in which the micro region or a predetermined region in the micro region is irradiated with the laser beam for a predetermined number of times; and generating mass spectrometry data indicating a mass spectrometry result for the mixed crystal, on the basis of ion detection signals acquired by the mass spectrometry device during the second laser irradiation.

公开(公告)号：US20230282310A1

公开(公告)日：2023-09-07

申请号：US17685453

申请日：2022-03-03

Applicant: SHIMADZU CORPORATION

Inventor： Yoshihiro YAMADA ,  Kanae TERAMOTO

IPC: G16B40/00 ,  H01J49/00

CPC classification number: G16B40/00 ,  H01J49/0036

Abstract: To enable a correct and easy discrimination of microorganisms, a microorganism discrimination method includes: acquiring mass spectra related to known microorganisms which belong to the same species and whose subspecies, strains or types are known (S11); retrieving a list describing m/z values of marker-candidate proteins which are supposed to vary in mass among different subspecies, strains or types (S12); creating a mask which gives non-zero values only within a predetermined range including each of the listed m/z values (S14); masking each of the mass spectra (S15); creating wavelet images by performing continuous wavelet transform on the mass spectra (S16); creating a discriminant model by machine learning using, as training data, the wavelet images and information of the subspecies, strains or types of the known microorganisms; and discriminating the subspecies, strain or type of an unknown microorganism by applying a mass spectrum of this microorganism to the discriminant model.

公开(公告)号：US20240120189A1

公开(公告)日：2024-04-11

申请号：US17960609

申请日：2022-10-05

Applicant: SHIMADZU CORPORATION

Inventor： Kanae TERAMOTO

IPC: H01J49/16 ,  H01J49/00 ,  H01J49/40

CPC classification number: H01J49/164 ,  H01J49/0036 ,  H01J49/40

Abstract: A method for analyzing MALDI mass spectrometry data includes: calculating m/z values respectively corresponding to peaks in amass spectrum, from data corresponding to the mass spectrum acquired by MALDI mass spectrometry performed on a sample; and performing at least one of calculations (a) and (b), based on the m/z values and information on kinds of ions possibly generated from the sample in the mass spectrometry, where calculation (a) includes assuming that ions respectively corresponding to the peaks are first ions singly charged, and calculating m/z of second ions respectively corresponding to the first ions and being multiply charged, and calculation (b) includes assuming that ions respectively corresponding to the peaks are first ions that are multiply charged ions, and calculating m/z of second ions respectively corresponding to the first ions and being singly charged.

公开(公告)号：US20200300863A1

公开(公告)日：2020-09-24

申请号：US16801162

申请日：2020-02-26

Applicant: SHIMADZU CORPORATION

Inventor： Kanae TERAMOTO

IPC: G01N33/68 ,  H01J49/04 ,  H01J49/00

Abstract: A microorganism identification method utilizing mass spectrometry is provided. More specifically, a method for identifying a phylotype of Cutibacterium acnes utilizing mass spectrometry is provided. The method includes a) a step for reading out a m/z value of a peak derived from a marker protein on a mass spectrum which is obtained by mass spectrometry of a sample containing microorganisms; and b) a step for judging whether the sample contains Cutibacterium acnes (C. acnes) based on the m/z value.

公开(公告)号：US20220068622A1

公开(公告)日：2022-03-03

申请号：US17399135

申请日：2021-08-11

Applicant: SHIMADZU CORPORATION

Inventor： Shinichi IWAMOTO ,  Kanae TERAMOTO

IPC: H01J49/00 ,  G16C20/20 ,  G01N33/68

Abstract: Provided is a display-processing device for mass spectrometry data capable of presenting a mass spectrum of a test microorganism and existing genome-related information so that the relationship between the two kinds of information can be easily understood. In the device, a spectrum acquirer (41) acquires a mass spectrum (80) of a test microorganism. A genome-related information acquirer (42) acquires genome-related information of a known microorganism which is identical or related to the test microorganism, based on the mass spectrum. A correspondence relationship determiner (43) determines a correspondence relationship between peaks on the mass spectrum and proteins expressed in the known microorganism. A display controller (45) displays, on a display device, identifiers (81) and a genome map (70) along with the mass spectrum, each identifier indicating what protein corresponds to a given peak, and the genome map showing the location of the gene encoding each protein on the genome.

公开(公告)号：US20210102953A1

公开(公告)日：2021-04-08

申请号：US17047997

申请日：2019-03-08

Applicant: SHIMADZU CORPORATION

Inventor： Kanae TERAMOTO

IPC: G01N33/68 ,  G01N27/62 ,  H01J49/04 ,  H01J49/16 ,  H01J49/00

Abstract: A mass spectrometry kit for use in mass spectrometry with ionizing a sample by matrix-assisted laser desorption/ionization includes: a matrix reagent in a solid state and/or an additive of matrix in a solid state; and a plurality of containers each containing the matrix reagent and/or the additive therein.