公开(公告)号：US20210087608A1

公开(公告)日：2021-03-25

申请号：US17024225

申请日：2020-09-17

Applicant: SHIMADZU CORPORATION

Inventor： Naoko TAKAOKA

IPC: C12Q1/686 ,  C12Q1/6806

Abstract: A method for testing target nucleic acid includes the steps from (1) through (5) below: (1) mixing a specimen containing target nucleic acid with positive control nucleic acid to obtain a specimen mixture of the specimen and the positive control nucleic acid; (2) mixing the specimen mixture with a PCR buffer solution containing a surfactant to obtain a buffer solution mixture; (3) adding a portion of the buffer mixture to a solid composition for PCR control containing DNA polymerase, positive control nucleic acid, and PCR reaction control nucleic acid; (4) adding a portion of the buffer mixture to a solid composition for PCR reaction containing DNA polymerase and one or more kinds of PCR primer pair; and (5) detecting a PCR product generated as a result of the steps (3) and (4).

公开(公告)号：US20210031205A1

公开(公告)日：2021-02-04

申请号：US16942843

申请日：2020-07-30

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Kenji NINOMIYA ,  Naoko TAKAOKA ,  Shinichiro KOBAYASHI

IPC: B01L9/00 ,  G01N35/10

Abstract: A dispensing tip holder is used when a dispensing tip is attached to a tip of a pipette, and includes a peripheral wall having a shape surrounding an outer peripheral surface of the dispensing tip, and an insertion portion having an insertion opening into which the dispensing tip is to be inserted. The peripheral wall has a first end into which the dispensing tip is to be inserted, and has a second end provided at a position opposite to the first end. The insertion portion is formed in the peripheral wall such that an outer peripheral surface of the dispensing tip abuts against an edge of the insertion opening, and is formed in the peripheral wall such that a tip of the dispensing tip does not project from the second end of the peripheral wall, when the dispensing tip is inserted into the insertion opening from the first end.

公开(公告)号：US20210114018A1

公开(公告)日：2021-04-22

申请号：US16657336

申请日：2019-10-18

Applicant: SHIMADZU CORPORATION

Inventor： Naoko TAKAOKA ,  Masamitsu SHIKATA ,  Kenji NINOMIYA ,  Shinichiro KOBAYASHI

IPC: B01L3/00 ,  G01N21/03 ,  G01N21/64

Abstract: A reaction vessel has plural wells each configured to accommodate a sample on the same plane or on the same straight line. Among the wells of a well plate, a specific well other than a well at a center of point symmetry is an orientation recognizing well in which a fluorescent dye is placed. The reaction vessel can identify a position of the orientation recognizing well by using fluorescence emitted from the fluorescent dye, and can thereby recognize an orientation of the reaction vessel.

公开(公告)号：US20190022658A1

公开(公告)日：2019-01-24

申请号：US16040966

申请日：2018-07-20

Applicant: SHIMADZU CORPORATION

Inventor： Kenji NINOMIYA ,  Masamitsu SHIKATA ,  Naoko TAKAOKA ,  Shinichiro KOBAYASHI

IPC: B01L7/00

Abstract: A gene measurement apparatus includes: a temperature control block that is provided with, on its upper surface, a plurality of reaction vessel wells each being used to house a reaction vessel, and that performs temperature treatment on a specimen in the reaction vessel by controlling temperature of the plurality of reaction vessel wells using a heating element or a cooling element; and a reaction vessel detector configured to detect whether the reaction vessel is housed in each of the plurality of reaction vessel wells of the temperature control block before the temperature treatment is started.

公开(公告)号：US20250059613A1

公开(公告)日：2025-02-20

申请号：US17912379

申请日：2021-03-25

Applicant: SHIMADZU CORPORATION

Inventor： Shinichiro KOBAYASHI ,  Masamitsu SHIKATA ,  Kenji NINOMIYA ,  Naoko TAKAOKA ,  Hideaki MARUSE

IPC: C12Q1/70 ,  C12Q1/6844

Abstract: A method for testing a novel coronavirus, the method including steps of: mixing a specimen sample collected from a subject, or a mixed liquid of the specimen sample and a medium, with a specimen treatment liquid containing sodium hydroxide as a main component, to obtain a mixed liquid; incubating the mixed liquid; adding a master mix containing a reaction liquid, an internal standard substance, a primer, a probe, a reverse transcriptase, and a PCR enzyme to the mixed liquid after the incubation, to obtain a final mixed liquid; subjecting the final mixed liquid to a reverse transcription reaction treatment; and detecting, through use of the probe, DNA amplified by PCR using the DNA generated by the reverse transcription reaction treatment as a template.

公开(公告)号：US20180199922A1

公开(公告)日：2018-07-19

申请号：US15405446

申请日：2017-01-13

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Naoko TAKAOKA

IPC: A61B10/00 ,  B01L3/00 ,  G01N1/08

CPC classification number: A61B10/0038 ,  B01L3/50825 ,  B01L2200/025 ,  B01L2300/042

Abstract: A stool collection container includes: a stool collection container main body having a structure in which a stick holder for holding a stool collection stick is detachably mounted on one end side while the stool collection part and the stool collection stick are housed inside; and a collection needle penetrating through a wall surface of the another end side of the stool collection container main body so that a tip thereof is inserted into the stool collection container main body, the collection needle being provided so as to be detachable from the stool collection container main body. When the stick holder is mounted on one end side of the stool collection container main body, the tip of the collection needle is inserted into the opening of the stool collection part housed inside the stool collection container main body.