公开(公告)号：US11454588B2

公开(公告)日：2022-09-27

申请号：US17142632

申请日：2021-01-06

申请人： SONY CORPORATION

发明人： Masaya Kakuta ,  Koji Futamura ,  Yoshitsugu Sakai ,  Yasunobu Kato

IPC分类号： G01N21/27 ,  G01N15/14 ,  G01N21/64 ,  G01N15/00

摘要： A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.

公开(公告)号：US10656090B2

公开(公告)日：2020-05-19

申请号：US16295519

申请日：2019-03-07

申请人： Sony Corporation

发明人： Yasunobu Kato ,  Yoshitsugu Sakai

IPC分类号： G01J3/44 ,  G01N21/64

摘要： A fluorescence intensity calculating apparatus, includes a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors, and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.

公开(公告)号：US11156544B2

公开(公告)日：2021-10-26

申请号：US16782383

申请日：2020-02-05

申请人： Sony Corporation

发明人： Yuya Suzuki ,  Fumitaka Otsuka ,  Masashi Kimoto ,  Shingo Imanishi ,  Yoshitsugu Sakai

IPC分类号： G01N21/00 ,  G01N15/14 ,  G01N15/10

摘要： The present technology provides a technology for stabilizing break-off timings. Therefore, according to the present technology, there is provided a microparticle analysis device or the like including at least: a flow path in which a fluid including a sample flow containing microparticles and a sheath flow flowing to contain the sample flow; a droplet formation unit configured to form a droplet in the fluid by imparting vibration to the fluid using a vibration element; an electric charge application unit configured to apply electric charge to a droplet containing the microparticles; an imaging unit configured to obtain a photo of a phase of a certain time; and a control unit configured to control a timing at which the droplet breaks off on a basis of the photo.

公开(公告)号：US11135585B2

公开(公告)日：2021-10-05

申请号：US16305768

申请日：2017-03-07

申请人： SONY CORPORATION

发明人： Yoshitsugu Sakai ,  Ichiro Fujii ,  Ryoichi Kubota ,  Shinichi Hasegawa ,  Takashi Miyata ,  Emi Ikeda

IPC分类号： B01L3/00

摘要： The present technology is mainly directed to providing a technology that enables smooth setting of sheath liquid. Provided is a fluid control device including at least a support portion that supports a sheath liquid storage unit and a sealed portion that houses the support portion. The support portion is detachable from the sealed portion, and the sealed portion is controlled by pressurization in order to feed sheath liquid stored in the sheath liquid storage unit to a microparticle measurement device.

公开(公告)号：US20140365159A1

公开(公告)日：2014-12-11

申请号：US14452085

申请日：2014-08-05

申请人： Sony Corporation

发明人： Yasunobu Kato ,  Yoshitsugu Sakai

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6439

摘要： A fluorescence intensity calculating apparatus, includes a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors, and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.

摘要翻译： 一种荧光强度计算装置，包括测量部分，其被配置为接收由多个荧光染料产生的荧光，该荧光染料被激发，所述荧光染料被多个荧光染料激发，所述微粒被多个荧光染料多个标记，所述荧光染料具有与对应于不同接收光的光电检测器彼此重叠的荧光波长带 波长带，其数量大于荧光染料的数量，并且通过从光电检测器收集检测值来获得测量的光谱，以及计算部件，被配置为基于获得的单染色光谱的线性和近似测量的光谱 从分别用荧光染料标记的微粒分别计算由荧光染料产生的荧光的强度。

公开(公告)号：US10908075B2

公开(公告)日：2021-02-02

申请号：US16100884

申请日：2018-08-10

申请人： SONY CORPORATION

发明人： Masaya Kakuta ,  Koji Futamura ,  Yoshitsugu Sakai ,  Yasunobu Kato

IPC分类号： G01N21/27 ,  G01N15/14 ,  G01N21/64 ,  G01N15/00

摘要： A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.

公开(公告)号：US20170186198A1

公开(公告)日：2017-06-29

申请号：US15460493

申请日：2017-03-16

申请人： Sony Corporation

发明人： Yoshitsugu Sakai

IPC分类号： G06T11/20 ,  G01N21/64 ,  G06T11/00 ,  G01N15/14

CPC分类号： G06T11/206 ,  G01N15/1463 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6421 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06T11/001

摘要： Disclosed herein is a microparticle analyzing apparatus including a detecting portion configured to simultaneously detect a fluorescence generated from a microparticle in plural wavelength regions and a displaying portion configured to display thereon detection results in the plural wavelength regions in a form of a spectrum.

公开(公告)号：US11340167B2

公开(公告)日：2022-05-24

申请号：US16848115

申请日：2020-04-14

申请人： Sony Corporation

发明人： Yasunobu Kato ,  Yoshitsugu Sakai

IPC分类号： G01N21/64

摘要： A fluorescence intensity calculating apparatus, includes a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors, and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.

公开(公告)号：US11112345B2

公开(公告)日：2021-09-07

申请号：US16305885

申请日：2017-03-07

申请人： SONY CORPORATION

发明人： Yoshitsugu Sakai ,  Masaaki Abe ,  Koichi Tsukihara ,  Shoji Akiyama ,  Shinichi Hasegawa

IPC分类号： G01N15/14

摘要： Provided is a microparticle measurement device including a light emission unit that emits light to a microparticle to be analyzed and a light detection unit that detects light generated from the microparticle at a predetermined detection position. The microparticle measurement device further includes an analysis unit that is connected to the light detection unit and analyzes a detection value of the light detected by the light detection unit. The light detection unit is movable from the detection position.

公开(公告)号：US11074727B2

公开(公告)日：2021-07-27

申请号：US16184616

申请日：2018-11-08

申请人： Sony Corporation

发明人： Yoshitsugu Sakai

IPC分类号： G01N15/14 ,  G01N21/64 ,  G06T11/00 ,  G06T11/20

摘要： Disclosed herein is a microparticle analyzing apparatus including a detecting portion configured to simultaneously detect a fluorescence generated from a microparticle in plural wavelength regions and a displaying portion configured to display thereon detection results in the plural wavelength regions in a form of a spectrum.