公开(公告)号：US20160130642A1

公开(公告)日：2016-05-12

申请号：US14981366

申请日：2015-12-28

Applicant: STMicroelectronics S.R.L.

Inventor： Enrico Alessi ,  Daniele Ricceri

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2561/113 ,  C12Q2527/107

Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber, accommodating an array of nucleic acid probes at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution into the reaction chamber, wherein the solution contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes and of the primers so that a hybridization temperature of the probes is higher than an annealing temperature of the primers, whereby hybridization and annealing take place in respective separate temperature ranges.

Abstract translation: 一种用于进行核酸扩增的方法，包括提供反应室，在各个位置容纳核酸探针阵列，用于与各自的靶核酸杂交; 并将溶液引入所述反应室中，其中所述溶液含有能够结合靶核酸，核苷酸，核酸延伸酶和包含核酸的样品的引物。 核酸探针和引物的结构使得探针的杂交温度高于引物的退火温度，由此杂化和退火在相应的单独的温度范围内进行。

公开(公告)号：US11015218B2

公开(公告)日：2021-05-25

申请号：US14981366

申请日：2015-12-28

Applicant: STMicroelectronics S.R.L.

Inventor： Enrico Alessi ,  Daniele Ricceri

IPC: C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6844

Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber, accommodating an array of nucleic acid probes at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution into the reaction chamber, wherein the solution contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes and of the primers so that a hybridization temperature of the probes is higher than an annealing temperature of the primers, whereby hybridization and annealing take place in respective separate temperature ranges.