公开(公告)号：US20130224744A1

公开(公告)日：2013-08-29

申请号：US13856473

申请日：2013-04-04

Applicant: STMicroelectronics S.r.I

Inventor： Lucio RENNA ,  Clelia Carmen GALATI ,  Natalia Maria Rita SPINELLA

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/18 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2563/107 ,  C12Q2563/143

Abstract: A method for real-time quantitative detection of single-type, target nucleic acid sequences amplified using a PCR in a microwell, comprising introducing in the microwell a sample comprising target nucleic acid sequences, magnetic primers, and labelling probes; performing an amplification cycle to form labelled amplicons; attracting the magnetic primers to a surface through a magnetic field to form a layer including labelled amplification products and free magnetic primers; and detecting the labelled amplification products in the layer with a surface-specific reading method.