公开(公告)号：US10227620B2

公开(公告)日：2019-03-12

申请号：US15011855

申请日：2016-02-01

Applicant: SYMRISE AG

Inventor： Gerhard Krammer ,  Jakob Peter Ley ,  Katrin Geißler ,  Torsten Geißler ,  Frauke Gomoll ,  Peter Welters ,  Guido Jach ,  Ludger Wessjohann

IPC: C12N9/10 ,  C12P7/22 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  A23L27/20 ,  A23L27/24 ,  C12P13/00 ,  C12P13/02

Abstract: The invention relates to providing both fermentative and biotechnological methods for producing 3,4-methylized cinnamic acids, 3,4-methylized cinnamic acid esters, 3,4-dimethoxyphenethylamine, and 4-methylized cinnamic acid amides using a 4′-O-methyltransferase, optionally in combination with further enzymes, wherein the enzymes are selected by means of metabolic engineering and operation have been adapted by targeted optimization, and compositions obtained by means of the method.The invention further relates to vector systems, recombinant microorganisms or fungi, and specific nucleic acid segments and polypeptides.