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    METHOD OF SELECTING CORN EMBRYOS
    1.
    发明申请
    METHOD OF SELECTING CORN EMBRYOS 审中-公开

    公开(公告)号：US20190116746A1

    公开(公告)日：2019-04-25

    申请号：US15743191

    申请日：2016-07-06

    申请人： Syngenta Participations AG

    发明人： William Paul BULLOCK ,  Ulrich Stephan HANNAPPEL ,  Katie Marie Hunter

    IPC分类号： A01H1/04 ,  C12Q1/6895 ,  C12N15/10

    摘要： The present invention relates to a viability-retaining method of selecting corn embryos having a desired trait, said method comprising extracting embryos, removing associated non-embryogenic tissue or DNA from the exterior of the embryo; and extracting DNA directly from the embryo. The invention surprisingly shows that germinating embryos sampled directly for their DNA content are capable of continuing the germination process to form normal seedlings.

    HAPLOID INDUCTION COMPOSITIONS AND METHODS FOR USE THEREFOR
    3.
    发明申请
    HAPLOID INDUCTION COMPOSITIONS AND METHODS FOR USE THEREFOR 有权
    HAPLOID感应组合物及其使用方法

    公开(公告)号：US20170067067A1

    公开(公告)日：2017-03-09

    申请号：US14212504

    申请日：2014-03-14

    申请人： SYNGENTA PARTICIPATIONS AG

    发明人： Satya CHINTAMANANI ,  Brent DELZER ,  Michael L NUCCIO ,  Robert Arthur DIETRICH ,  Suresh Babu KADARU ,  Todd Lee WARNER ,  William Paul BULLOCK ,  Timothy KELLIHER

    IPC分类号： C12N15/82 ,  C12Q1/68

    CPC分类号： C12N15/8216 ,  A01H1/04 ,  A01H1/08 ,  C12N9/18 ,  C12N15/8218 ,  C12N15/8261 ,  C12N15/8287 ,  C12Q1/686 ,  C12Q1/6895 ,  Y02A40/146

    摘要： Provided are isolated cDNAs. In some embodiments, the isolated cDNA are selected from the group consisting of: (a) a nucleic acid having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53, optionally wherein the percent identity is calculated over the entire length of SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53; (b) a nucleic acid having at least 95% identity over nucleotides 91-1452 of SEQ ID NO: 33; (c) a nucleic acid that is the reverse complement of either of (a) or (b); and (d) a nucleic acid that encodes a polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 54, or SEQ ID NO: 55, or SEQ ID NO: 56, or SEQ ID NO: 57 optionally wherein the percent identity is calculated over the entire length of SEQ ID NO: 54, or SEQ ID NO: 55, or SEQ ID NO: 56, or SEQ ID NO: 57.Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid—inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

    摘要翻译： 还提供了表达盒; 向量 转基因植物细胞; 植物，植物部分和种子; 分离的多肽; 目前公开的核酸的扩增子和信息片段; 包括扩增引物对的组合物; 生产展示HI的植物的方法; 用于鉴定植物中与HI相关的等位基因的存在或不存在的方法; 将单倍体诱导核苷酸序列插入植物的方法; 以及用于选择预期用表现单倍体诱导性状的植物产生后代的亲本植物的方法。