公开(公告)号：US20080193986A1

公开(公告)日：2008-08-14

申请号：US11712494

申请日：2007-03-01

申请人： Sang Young Kim ,  Do Sun Kim ,  Hyung Moo Jung ,  Jung Kul Lee

发明人： Sang Young Kim ,  Do Sun Kim ,  Hyung Moo Jung ,  Jung Kul Lee

IPC分类号： C12P17/18 ,  C12N1/20

CPC分类号： C12R1/04 ,  C12N1/20 ,  C12P19/44 ,  C12P19/46

摘要： The present invention provides a process for preparing vancomycin hydrochloride using a mutant strain of Amycolatopsis orientalis (accession No. KCCM-10836P) comprising the steps of i) mutating and isolating a strain of Amycolatopsis orientalis (accession No. KCCM-10836P) for producing vancomycin from mother strain of Amycolatopsis orientalis (accession No. ATCC-19795) using NTG(N-methyl-N′-nitro-N-nitrosoguanidine) in the selection medium; ii) seed culturing the isolated mutant strain of Amycolatopsis orientalis (accession No. KCCM-10836P); iii) cultivating and fermenting said mutant strain of Amycolatopsis orientalis in the fermentation medium consisting of 12˜18 (w/v) % of dextrin, 2.2˜3.8 (w/v) % of bean powder, 1.9˜2.9 (w/v) % of potato protein, 0.10˜0.14 (w/v) % of sodium chloride and a small amount of minerals; iv) filtering vancomycin using microfilter in the cultivation broth by removing mycelia; v) purifying obtained vancomycin using column system compacted with cation exchange resin, anion exchange resin and absorbent resin; and vi) crystallizing the purified vancomycin with hydrochloric acid.

摘要翻译： 本发明提供一种使用东方异a螨突变菌株（登录号KCCM-10836P）制备万古霉素盐酸盐的方法，其包括以下步骤：i）突变和分离用于产生万古霉素的侧寄生菌株（登录号KCCM-10836P） 从选择培养基中使用NTG（N-甲基-N'-硝基-N-亚硝基胍）的来自东方的抗菌纲菌株（登录号ATCC-19795） ii）种子培养分离的东方异基因突变菌株（登录号KCCM-10836P）; iii）在由12〜18（w / v）％的糊精，2.2〜3.8（w / v）％的豆粉，1.9〜2.9（w / v）的糊状物组成的发酵培养基中，培养和发酵所述的东方双歧杆菌突变菌株， ％的土豆蛋白，0.10〜0.14（w / v）的氯化钠和少量的矿物质; iv）通过去除菌丝体在培养液中使用微过滤器过滤万古霉素; v）使用用阳离子交换树脂，阴离子交换树脂和吸收树脂压实的柱系统纯化得到的万古霉素; 和vi）用盐酸结晶纯化的万古霉素。