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    Mutant strain of Amycolatopsis orientalis and process for preparing vancomycin hydrochloride
    1.
    发明申请
    Mutant strain of Amycolatopsis orientalis and process for preparing vancomycin hydrochloride 审中-公开
    Amycolatopsis orientalis的突变株及其制备万古霉素盐酸盐的方法

    公开(公告)号：US20080193986A1

    公开(公告)日：2008-08-14

    申请号：US11712494

    申请日：2007-03-01

    申请人： Sang Young Kim ,  Do Sun Kim ,  Hyung Moo Jung ,  Jung Kul Lee

    发明人： Sang Young Kim ,  Do Sun Kim ,  Hyung Moo Jung ,  Jung Kul Lee

    IPC分类号： C12P17/18 ,  C12N1/20

    CPC分类号： C12R1/04 ,  C12N1/20 ,  C12P19/44 ,  C12P19/46

    摘要： The present invention provides a process for preparing vancomycin hydrochloride using a mutant strain of Amycolatopsis orientalis (accession No. KCCM-10836P) comprising the steps of i) mutating and isolating a strain of Amycolatopsis orientalis (accession No. KCCM-10836P) for producing vancomycin from mother strain of Amycolatopsis orientalis (accession No. ATCC-19795) using NTG(N-methyl-N′-nitro-N-nitrosoguanidine) in the selection medium; ii) seed culturing the isolated mutant strain of Amycolatopsis orientalis (accession No. KCCM-10836P); iii) cultivating and fermenting said mutant strain of Amycolatopsis orientalis in the fermentation medium consisting of 12˜18 (w/v) % of dextrin, 2.2˜3.8 (w/v) % of bean powder, 1.9˜2.9 (w/v) % of potato protein, 0.10˜0.14 (w/v) % of sodium chloride and a small amount of minerals; iv) filtering vancomycin using microfilter in the cultivation broth by removing mycelia; v) purifying obtained vancomycin using column system compacted with cation exchange resin, anion exchange resin and absorbent resin; and vi) crystallizing the purified vancomycin with hydrochloric acid.

    摘要翻译： 本发明提供一种使用东方异a螨突变菌株（登录号KCCM-10836P）制备万古霉素盐酸盐的方法，其包括以下步骤：i）突变和分离用于产生万古霉素的侧寄生菌株（登录号KCCM-10836P） 从选择培养基中使用NTG（N-甲基-N'-硝基-N-亚硝基胍）的来自东方的抗菌纲菌株（登录号ATCC-19795） ii）种子培养分离的东方异基因突变菌株（登录号KCCM-10836P）; iii）在由12〜18（w / v）％的糊精，2.2〜3.8（w / v）％的豆粉，1.9〜2.9（w / v）的糊状物组成的发酵培养基中，培养和发酵所述的东方双歧杆菌突变菌株， ％的土豆蛋白，0.10〜0.14（w / v）的氯化钠和少量的矿物质; iv）通过去除菌丝体在培养液中使用微过滤器过滤万古霉素; v）使用用阳离子交换树脂，阴离子交换树脂和吸收树脂压实的柱系统纯化得到的万古霉素; 和vi）用盐酸结晶纯化的万古霉素。

    Dual cofactor specific ribitol dehydrogenase and use thereof
    2.
    发明授权
    Dual cofactor specific ribitol dehydrogenase and use thereof 有权
    双辅因子特异性核糖醇脱氢酶及其用途

    公开(公告)号：US09005937B2

    公开(公告)日：2015-04-14

    申请号：US13880374

    申请日：2012-01-27

    申请人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    发明人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    IPC分类号： C12P19/02 ,  C12N9/00 ,  C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P19/02 ,  C12N9/0006 ,  C12Y101/01056

    摘要： There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing L-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing L-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from Zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique.

    摘要翻译： 提供了新的核糖醇脱氢酶，确定双辅酶特异性的残基，以及使用其制备L-核酮糖的方法，更具体地，涉及制备核糖醇脱氢酶的稀有糖，编码它的核酸分子，包含 核酸分子，包含载体的转化体，核糖醇脱氢酶的突变体和使用核糖醇脱氢酶制备L-核酮糖的方法。 具有双辅酶特异性的衍生自运动发酵单胞菌的核糖醇脱氢酶可以有效地用于制备高价稀有糖，并且对所有的脱氢酶作为基础技术应用于核糖醇脱氢酶的辅酶特异性决定簇的研究。

    DUAL COFACTOR SPECIFIC RIBITOL DEHYDROGENASE AND USE THEREOF
    3.
    发明申请
    DUAL COFACTOR SPECIFIC RIBITOL DEHYDROGENASE AND USE THEREOF 有权
    双重特异性RIBITOL DEHYDROGENASE及其用途

    公开(公告)号：US20140242642A1

    公开(公告)日：2014-08-28

    申请号：US13880374

    申请日：2012-01-27

    申请人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    发明人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    IPC分类号： C12P19/02 ,  C12N9/04

    CPC分类号： C12P19/02 ,  C12N9/0006 ,  C12Y101/01056

    摘要： There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing L-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing L-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from Zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique.

    摘要翻译： 提供了新的核糖醇脱氢酶，确定双辅酶特异性的残基，以及使用其制备L-核酮糖的方法，更具体地，涉及制备核糖醇脱氢酶的稀有糖，编码它的核酸分子，包含 核酸分子，包含载体的转化体，核糖醇脱氢酶的突变体和使用核糖醇脱氢酶制备L-核酮糖的方法。 具有双辅酶特异性的衍生自运动发酵单胞菌的核糖醇脱氢酶可以有效地用于制备高价稀有糖，并且对所有的脱氢酶作为基础技术应用于核糖醇脱氢酶的辅酶特异性决定簇的研究。