公开(公告)号：US20090075352A1

公开(公告)日：2009-03-19

申请号：US11722632

申请日：2005-05-23

申请人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee ,  Sang Jun Lee

发明人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee ,  Sang Jun Lee

IPC分类号： C12P7/46 ,  G06G7/48 ,  C12N1/21

CPC分类号： C12P7/46 ,  C12N9/1205 ,  C12N15/1089

摘要： The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced.

摘要翻译： 本发明涉及一种基于在计算机分析中改进菌株的方法，其中将用于产生有用物质的靶菌株的基因组信息与过量产生有用物质的菌株的基因组信息进行比较，以便 主要是用于过量生产有用物质的筛选基因，然后通过用代谢通量分析进行模拟来次要筛选待缺失的基因。 根据本发明，可以通过代谢和基因工程方法有效地构建改进的菌株，其包括比较分析用于产生有用物质的靶菌株的基因组信息和产生大量有用物质的菌株的基因组信息 筛选候选基因并在筛选的候选基因的计算机模拟中进行选择要缺失的基因的组合，其显示有用物质的生产的改进。 因此，可以显着降低实际湿测试所需的时间，精力和成本。

公开(公告)号：US07752540B2

公开(公告)日：2010-07-06

申请号：US11148907

申请日：2005-06-09

申请人： Sang Yup Lee ,  Hong Seok Yun ,  Dong Yup Lee ,  Seung Hyun Lee ,  Joon Woo Jeong ,  Tae Yong Kim

发明人： Sang Yup Lee ,  Hong Seok Yun ,  Dong Yup Lee ,  Seung Hyun Lee ,  Joon Woo Jeong ,  Tae Yong Kim

IPC分类号： G06F17/00

CPC分类号： G06F19/26 ,  G06F19/12

摘要： The present invention relates to an MFA (Metabolic Flux Analysis) information system using an XML (eXtensible Markup Language) and an operating method thereof. More specifically, the invention relates to an MFA information system and an operating method thereof, which generates, edits, stores and visualizes an MFA model feature and an MFA object using XML, and edits, stores and visualizes the result obtained by performing MFA based on the object. The present invention provides the MFA information system and method capable of generating, editing, storing and visualizing MFA model features and MFA objects using XML. Accordingly, MFA can be easily performed by utilizing advantages of XML, such as transplantation, reusability, deciphering, scalability, flexibility and effective data exchange, and thus the present invention can be applied to cell improvement using metabolic engineering.

摘要翻译： 本发明涉及使用XML（可扩展标记语言）的MFA（代谢通量分析）信息系统及其操作方法。 更具体地，本发明涉及一种MFA信息系统及其操作方法，其使用XML生成，编辑，存储和可视化MFA模型特征和MFA对象，并且通过执行MFA获得的结果进行编辑，存储和可视化 物体。 本发明提供了能够使用XML来生成，编辑，存储和可视化MFA模型特征和MFA对象的MFA信息系统和方法。 因此，通过利用移植，可重用性，解密，可扩展性，灵活性和有效的数据交换等XML的优点，可以容易地实现MFA，因此本发明可以应用于使用代谢工程的细胞改良。

公开(公告)号：US20090215048A1

公开(公告)日：2009-08-27

申请号：US11994330

申请日：2005-10-14

申请人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee

发明人： Sang Yup Lee ,  Tae Yong Kim ,  Dong Yup Lee

IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12N15/00 ,  C12N15/74 ,  C12P7/46

CPC分类号： G16B5/00 ,  G16B20/00

摘要： The present invention relates to an in silico method for improving an organism on the basis of the flux sum (φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.

摘要翻译： 本发明涉及一种基于代谢物的通量和（phi）改善生物体的计算机方法，更具体地涉及一种筛选提高有用物质产量的关键代谢物的方法，该方法包括定义 用于产生有用物质的生物体的代谢物利用作为助熔剂和扰乱通量和，以及改善产生有用物质的生物体的方法，所述方法包括删除和/或扩增与上述筛选的关键代谢物相关的基因 。 根据本发明，可以准确地预测特定代谢物和有用物质生产之间的相关性，从而可以通过引入和/或扩增和/或删除表达与...相关的酶的基因来开发具有增加的有用物质生产的生物体 特异性代谢物。 此外，还可以通过在培养期间加入特定的代谢物来增加有用物质的产生。