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    Dimers of unsymmetrical cyanine dyes
    4.
    发明授权
    Dimers of unsymmetrical cyanine dyes 失效
    不对称花青染料的二聚体

    公开(公告)号：US5582977A

    公开(公告)日：1996-12-10

    申请号：US180763

    申请日：1994-01-06

    申请人： Stephen T. Yue ,  Iain D. Johnson ,  Richard P. Haugland

    发明人： Stephen T. Yue ,  Iain D. Johnson ,  Richard P. Haugland

    IPC分类号： C09B23/02 ,  G01N33/52 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C09B23/02 ,  G01N33/52

    摘要： The invention relates to dimers of unsymmetrical cyanine dyes, typically dimers of benzthiazole or benzoxazole derivatives, that exhibit enhanced fluorescence on binding with DNA or RNA, The dimers generally have the formula: ##STR1## where R.sup.1 and R.sup.2, which may be the same or different, are alkyl groups having 1-6 carbons;X is O, S, or N--R.sup.3, where R.sup.3 is H or an alkyl group having 1-6 carbons;Z is O, S, or N--R.sup.4, where R.sup.4 is H or an alkyl group having 1-6 carbons;n and s, which may be the same or different, =0, 1, or 2;Y is HC.dbd.CH; andp, m, q, and r=0 or 1, such that p+m=1 and q+r=1; andwhere -BRIDGE- has the general formula:--(CH.sub.2).sub..alpha. --[A.sup.1 --(CH.sub.2).sub..beta. --].sub.I [A.sup.2 --(CH.sub.2).sub..gamma. --].sub.II A.sup.3 --(CH.sub.2).sub..delta. --where .alpha., .beta., .gamma., and .delta., which may be the same or different, are integers greater than 1 and less than 5;I and II, which may be the same or different, =0 or 1; andA.sup.1, A.sup.2, and A.sup.3, which may be the same or different, are independently O; S; (CH.sub.2).sub..mu. where .mu.=0 or 1; --(NR.sup.5)-- where R.sup.5 is H or an alkyl group having 1-6 carbons; or--(N.sup.+ R.sup.6 R.sup.7)-- where R.sup.6 and R.sup.7, which may be the same or different, are independently hydrogen or an alkyl group having 1-6 carbons.

    摘要翻译： 本发明涉及不对称花青染料的二聚体，通常是苯并噻唑或苯并恶唑衍生物的二聚体，其在与DNA或RNA结合时表现出增强的荧光。二聚体通常具有下式：具有下式的烷基：具有 1-6个碳; X是O，S或N-R 3，其中R 3是H或具有1-6个碳的烷基; Z是O，S或N-R 4，其中R 4是H或具有1-6个碳的烷基; n和s可以相同或不同，= 0,1或2; Y为HC = CH; 和p，m，q和r = 0或1，使得p + m = 1和q + r = 1; 并且其中-BRIDGE具有以下通式： - （CH 2）a - [A 1-（CH 2）β - ] I [A2-（CH 2）γ - ] IIA 3 - （CH 2）δ-其中α，β，γ和 δ可以相同或不同，是大于1且小于5的整数; I和II可以相同或不同，= 0或1; 和可以相同或不同的A1，A2和A3独立地为O; S; （CH 2）m，其中mu = 0或1; - （NR5） - 其中R5是H或具有1-6个碳原子的烷基; 或 - （N + R6R7） - 其中R6和R7可以相同或不同，独立地为氢或具有1-6个碳原子的烷基。

    Unsymmetrical cyanine dyes with a cationic side chain
    5.
    发明授权
    Unsymmetrical cyanine dyes with a cationic side chain 失效
    具有阳离子侧链的不对称花青染料

    公开(公告)号：US5321130A

    公开(公告)日：1994-06-14

    申请号：US833006

    申请日：1992-02-10

    申请人： Stephen T. Yue ,  Iain D. Johnson ,  Zhijian Huang ,  Richard P. Haugland

    发明人： Stephen T. Yue ,  Iain D. Johnson ,  Zhijian Huang ,  Richard P. Haugland

    IPC分类号： C09B23/02 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C09B23/02 ,  Y10S435/968 ,  Y10S436/80

    摘要： The invention relates to unsymmetrical cyanine dyes with a cationic side chain, typically benzothiazole or benzoxazole derivatives, that exhibit enhanced fluorescence on binding with DNA or RNA, where such fluorescence can be used for evaluating the presence of nucleic acid polymers. The dyes generally have the formula: ##STR1## where R.sup.1 is an alkyl group having 1-6 carbons; X is 0, S, or NR.sup.2, where R.sup.2 is an alkyl group having 1-6 carbons, or CR.sup.3 R.sup.4, where R.sup.3 and R.sup.4, which may be the same or different, are alkyl groups having 1-6 carbons;n=0, 1, or 2;Z.sup.1 and Z.sup.2, which may be the same or different, are independently hydrogen, an alkyl group having 1-6 carbons, or aryl, or Z.sup.1 and Z.sup.2 taken in combination complete a 6-membered aromatic ring;Y is HC.dbd.CH; andeach of P and M=0 or 2, such that P+M=1; andTAIL is an aminoalkyl chain containing a backbone of 3-42 carbons and 1-5 positively charged nitrogens intermittently or equally spaced within the backbone, such that there are at least two carbons between sequential nitrogens.

    摘要翻译： 本发明涉及具有阳离子侧链（通常为苯并噻唑或苯并恶唑衍生物）的不对称花青染料，其在与DNA或RNA结合时表现出增强的荧光，其中该荧光可用于评估核酸聚合物的存在。 染料通常具有下式：其中R1是具有1-6个碳原子的烷基; X是0，S或NR2，其中R2是具有1-6个碳原子的烷基，或CR3R4，其中R3和R4可以相同或不同，是具有1-6个碳原子的烷基; n = 0,1或2; Z 1和Z 2可以相同或不同，独立地为氢，具有1-6个碳的烷基或芳基，或Z 1和Z 2组合形成6元芳环; Y为HC = CH; 并且P和M中的每一个= 0或2，使得P + M = 1; 并且TAIL是含有3-42个碳原子的主链和1-5个带正电荷的氮在主链内间歇或等间隔的氨基烷基链，使得在相继的氮之间存在至少两个碳。