利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

80 条记录 (耗时 0.037 秒)

    Enzyme substrate comprising a functional dye and associated technology and methods
    2.
    发明授权
    Enzyme substrate comprising a functional dye and associated technology and methods 有权
    酶底物包含功能染料及相关技术和方法

    公开(公告)号：US08778627B2

    公开(公告)日：2014-07-15

    申请号：US13326009

    申请日：2011-12-14

    申请人： Fei Mao ,  Hui Cen ,  Wai-Yee Leung

    发明人： Fei Mao ,  Hui Cen ,  Wai-Yee Leung

    IPC分类号： C12Q1/40 ,  C12Q1/48 ,  C12Q1/37 ,  C07D263/60

    CPC分类号： C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

    摘要： Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

    摘要翻译： 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或诊断疾病。

    Detection using a dye and a dye modifier
    5.
    发明授权
    Detection using a dye and a dye modifier 有权
    使用染料和染料改性剂进行检测

    公开(公告)号：US08524503B2

    公开(公告)日：2013-09-03

    申请号：US13413352

    申请日：2012-03-06

    申请人： Fei Mao ,  Hye Eun Hoover ,  Wai-Yee Leung

    发明人： Fei Mao ,  Hye Eun Hoover ,  Wai-Yee Leung

    IPC分类号： G01N33/00

    CPC分类号： C09B67/0033 ,  C09B69/02 ,  Y10T436/143333

    摘要： The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

    摘要翻译： 本发明一般涉及染料。 本发明提供了广泛的含有该染料和试剂盒的染料和试剂盒，其适用于标记各种生物分子如核酸，细胞和微生物。 本发明还提供了用于研究和开发，法医鉴定，环境研究，诊断，预后和/或治疗疾病状况的各种方法。

    ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS
    8.
    发明申请
    ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS 有权
    包含功能性染色及其相关技术和方法的酶基质

    公开(公告)号：US20120115129A1

    公开(公告)日：2012-05-10

    申请号：US13326009

    申请日：2011-12-14

    申请人： FEI MAO ,  HUI CEN ,  WAI-YEE LEUNG

    发明人： FEI MAO ,  HUI CEN ,  WAI-YEE LEUNG

    IPC分类号： C12Q1/37 ,  C12Q1/68 ,  C12Q1/25 ,  C07K17/02 ,  C07D413/06

    CPC分类号： C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

    摘要： Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

    摘要翻译： 提供了本发明的酶底物和相关技术。 本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。 酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。 通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。 因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物 ，或疾病的诊断。