公开(公告)号：US20240033377A1

公开(公告)日：2024-02-01

申请号：US18266076

申请日：2021-12-09

Applicant: Scribe Therapeutics Inc.

Inventor： Manuel MOHR ,  Katherine BANEY ,  Angus SIDORE ,  Cécile FORTUNY ,  Maroof ADIL ,  Addison WRIGHT ,  Brett T. STAAHL ,  Sean HIGGINS ,  Benjamin OAKES ,  Suraj MAKHIJA ,  Sarah DENNY

IPC: A61K48/00 ,  C12N9/22 ,  C12N15/86 ,  C12N15/11

CPC classification number: A61K48/005 ,  C12N9/22 ,  C12N15/86 ,  C12N15/11 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: Provided herein polynucleotides configured for incorporation into recombinant adeno-associated virus (AAV) vectors. The polynucleotides encode for CRISPR proteins, gRNA, and ancillary components of AAV vectors useful in the modification of target nucleic acids. The systems are also useful for introduction into cells, for example eukaryotic cells having mutations in the target nucleic acid of a gene. Also provided are methods of using such AAV vectors to modify cells having such mutations.

公开(公告)号：US20230054437A1

公开(公告)日：2023-02-23

申请号：US17572208

申请日：2022-01-10

Applicant: Scribe Therapeutics Inc.

Inventor： Gayathri VIJAYAKUMAR ,  Sean HIGGINS ,  Isabel COLIN ,  Sarah DENNY ,  Brett T. STAAHL ,  Benjamin OAKES ,  Angus SIDORE ,  Suraj MAKHIJA

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85

Abstract: Provided herein are engineered Class 2, Type V nucleases and guide RNAs useful for the editing of target nucleic acids. Also provided are methods of making and using such variants to modify nucleic acids.

公开(公告)号：US20240376462A1

公开(公告)日：2024-11-14

申请号：US18789158

申请日：2024-07-30

Applicant: Scribe Therapeutics Inc.

Inventor： Gayathri VIJAYAKUMAR ,  Sean HIGGINS ,  Isabel COLIN ,  Sarah DENNY ,  Brett T. STAAHL ,  Benjamin OAKES ,  Angus SIDORE ,  Suraj MAKHIJA

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12N15/86 ,  C12N15/90

Abstract: Provided herein are engineered Class 2, Type V nucleases and guide RNAs useful for the editing of target nucleic acids. Also provided are methods of making and using such variants to modify nucleic acids.

公开(公告)号：US20240026385A1

公开(公告)日：2024-01-25

申请号：US18039858

申请日：2021-12-02

Applicant: Scribe Therapeutics Inc.

Inventor： Gayathri VIJAYAKUMAR ,  Sean HIGGINS ,  Isabel COLIN ,  Sarah DENNY ,  Brett T. STAAHL ,  Benjamin OAKES ,  Angus SIDORE ,  Suraj MAKHIJA

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12N15/86

CPC classification number: C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  C12N15/111 ,  C12N15/86 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2320/50

Abstract: Provided herein are engineered Class 2, Type V nucleases and guide RNAs useful for the editing of target nucleic acids. Also provided are methods of making and using such variants to modify nucleic acids.

公开(公告)号：US20250011756A1

公开(公告)日：2025-01-09

申请号：US18663845

申请日：2024-05-14

Applicant: Scribe Therapeutics Inc.

Inventor： Gayathri VIJAYAKUMAR ,  Sean HIGGINS ,  Isabel COLIN ,  Sarah DENNY ,  Brett T. STAAHL ,  Benjamin OAKES ,  Angus SIDORE ,  Suraj MAKHIJA

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12N15/86 ,  C12N15/90

Abstract: Provided herein are engineered Class 2, Type V nucleases and guide RNAs useful for the editing of target nucleic acids. Also provided are methods of making and using such variants to modify nucleic acids.

公开(公告)号：US20240360474A1

公开(公告)日：2024-10-31

申请号：US18608127

申请日：2024-03-18

Applicant: Scribe Therapeutics Inc.

Inventor： Katherine BANEY ,  Isabel COLIN ,  Cécile FORTUNY ,  Sean HIGGINS ,  Suraj MAKHIJA ,  Brett T. STAAHL ,  Maroof ADIL ,  Benjamin OAKES ,  Addison WRIGHT ,  Angus SIDORE ,  Manuel MOHR ,  Sarah DENNY

IPC: C12N15/86 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/86 ,  C12N9/22 ,  C12N15/111 ,  C07K2319/09 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/531 ,  C12N2750/14122 ,  C12N2750/14123 ,  C12N2750/14143 ,  C12N2750/14152

Abstract: Provided herein are compositions and methods for use of self-inactivating recombinant vectors (SIRV) encoding Class 2 Type V and guide ribonucleic acid (gRNA) sequences useful for nucleic acid sequence editing, and including self-inactivating components. The SIRV may be delivered to cells as part of an AAV vector to target a gene of interest.

公开(公告)号：US20230183691A1

公开(公告)日：2023-06-15

申请号：US18164138

申请日：2023-02-03

Applicant: Scribe Therapeutics Inc.

Inventor： Jason FERNANDES ,  Sean HIGGINS ,  Isabel COLIN ,  Hannah SPINNER ,  Matthew GARDNER ,  Trent GOMBERG ,  Gayathri VIJAYAKUMAR ,  Sarah DENNY ,  Brett T. STAAHL ,  Maroof ADIL ,  Benjamin OAKES ,  Angus SIDORE ,  Suraj MAKHIJA

IPC: C12N15/11 ,  A61K9/00 ,  A61K9/50 ,  C07K14/005 ,  C12N9/22

CPC classification number: C12N15/111 ,  A61K9/0019 ,  A61K9/5052 ,  C07K14/005 ,  C12N9/22 ,  C12N2310/16 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2320/32

Abstract: Provided herein are delivery particle systems (XDP) useful for the delivery of payloads of any type. In some embodiments, a XDP particle system with tropism for target cells of interest is used to deliver CRISPR/Cas polypeptides (e.g., CasX proteins) and guide nucleic acids (gNA), for the modification of nucleic acids in target cells. Also provided are methods of making and using such XDP to modify the nucleic acids in such cells.