公开(公告)号：US20120003693A1

公开(公告)日：2012-01-05

申请号：US13211834

申请日：2011-08-17

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/00 ,  C12N1/15 ,  C12N9/00 ,  C12N1/19 ,  C07H21/04 ,  C12N1/21

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20080166761A1

公开(公告)日：2008-07-10

申请号：US11841151

申请日：2007-08-20

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N5/00

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20080274530A1

公开(公告)日：2008-11-06

申请号：US12141428

申请日：2008-06-18

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12N9/00

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20130011873A1

公开(公告)日：2013-01-10

申请号：US13611827

申请日：2012-09-12

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N15/63 ,  C12N9/00 ,  C12N1/19 ,  C12N1/15 ,  C12N15/52 ,  C12N1/21

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

公开(公告)号：US20080050773A1

公开(公告)日：2008-02-28

申请号：US11841211

申请日：2007-08-20

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

公开(公告)号：US20080171357A1

公开(公告)日：2008-07-17

申请号：US11940555

申请日：2007-11-15

申请人： Kenzo YOKOZEKI ,  Sonoko SUZUKI ,  Seiichi HARA ,  Isao ABE

发明人： Kenzo YOKOZEKI ,  Sonoko SUZUKI ,  Seiichi HARA ,  Isao ABE

IPC分类号： C12P21/00

CPC分类号： C07K5/06 ,  C12N9/16 ,  C12N9/52 ,  C12N9/80 ,  C12P21/02

摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

摘要翻译： 公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。 提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。 该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。