公开(公告)号：US08394940B2

公开(公告)日：2013-03-12

申请号：US12711719

申请日：2010-02-24

申请人： Yasuaki Takakura ,  Seiichi Hara ,  Toshiki Taba ,  Shunichi Suzuki ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Kenzo Yokozeki

发明人： Yasuaki Takakura ,  Seiichi Hara ,  Toshiki Taba ,  Shunichi Suzuki ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Kenzo Yokozeki

IPC分类号： C07H21/04

CPC分类号： C12P17/10 ,  C12N9/0022

摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

摘要翻译： 本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。 使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。 此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。

公开(公告)号：US08389241B2

公开(公告)日：2013-03-05

申请号：US13611827

申请日：2012-09-12

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US08329428B2

公开(公告)日：2012-12-11

申请号：US13211834

申请日：2011-08-17

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20120295304A1

公开(公告)日：2012-11-22

申请号：US13528126

申请日：2012-06-20

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/02

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

公开(公告)号：US08084227B2

公开(公告)日：2011-12-27

申请号：US11330076

申请日：2006-01-12

申请人： Kenzo Yokozeki ,  Isao Abe ,  Seiichi Hara

发明人： Kenzo Yokozeki ,  Isao Abe ,  Seiichi Hara

IPC分类号： C12P21/06 ,  C12P1/00 ,  C12N9/00 ,  C12N9/10

CPC分类号： C12P21/02

摘要： The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.

摘要翻译： 本发明提供了一种通过工业上有利且简单的途径以低成本获得的起始材料制备二肽的方法。 通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。

公开(公告)号：US20100317068A1

公开(公告)日：2010-12-16

申请号：US12836984

申请日：2010-07-15

申请人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

发明人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

IPC分类号： C12P13/06 ,  C07H21/00 ,  C12N9/10 ,  C12N1/21

CPC分类号： C12N9/1029 ,  C12P13/04

摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.

摘要翻译： 本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。 在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质 （B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物 。

公开(公告)号：US20100267082A1

公开(公告)日：2010-10-21

申请号：US12729656

申请日：2010-03-23

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/21 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20100221795A1

公开(公告)日：2010-09-02

申请号：US12711719

申请日：2010-02-24

申请人： YASUAKI TAKAKURA ,  SEIICHI HARA ,  TOSHIKI TABA ,  SHUNICHI SUZUKI ,  MASAKAZU SUGIYAMA ,  KUNIHIKO WATANABE ,  KENZO YOKOZEKI

发明人： YASUAKI TAKAKURA ,  SEIICHI HARA ,  TOSHIKI TABA ,  SHUNICHI SUZUKI ,  MASAKAZU SUGIYAMA ,  KUNIHIKO WATANABE ,  KENZO YOKOZEKI

IPC分类号： C12P17/10 ,  C07H21/00 ,  C12N1/21 ,  C12N9/02

CPC分类号： C12P17/10 ,  C12N9/0022

摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

摘要翻译： 本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。 使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。 此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。

公开(公告)号：US07749742B2

公开(公告)日：2010-07-06

申请号：US11940555

申请日：2007-11-15

申请人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06 ,  C12P13/04 ,  A61K38/00 ,  C07H21/04

CPC分类号： C07K5/06 ,  C12N9/16 ,  C12N9/52 ,  C12N9/80 ,  C12P21/02

摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

摘要翻译： 公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。 提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。 该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。

公开(公告)号：US07745182B2

公开(公告)日：2010-06-29

申请号：US12345922

申请日：2008-12-30

申请人： Hiroyuki Nozaki ,  Shinji Kuroda ,  Kunihiko Watanabe ,  Kenzo Yokozeki

发明人： Hiroyuki Nozaki ,  Shinji Kuroda ,  Kunihiko Watanabe ,  Kenzo Yokozeki

IPC分类号： C12P13/04 ,  C12N9/00 ,  C12N9/10

CPC分类号： C12P13/04 ,  C12N9/1014

摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.

摘要翻译： 提供了一种生产β-羟基氨基酸及其光学活性异构体的方法。 β-羟基氨基酸通过使预定的D-α-氨基酸和5,10-亚甲基四氢叶酸在衍生自属于副球菌属，氨基杆菌属或Ensifer属的微生物的酶存在下反应来制备。