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公开(公告)号：US06989456B2
公开(公告)日：2006-01-24
申请号：US10381174
申请日：2001-09-26
申请人： Naoko Seo , Shoji Kaneko , Fumi Sato , Seiji Norinobu , Mitsumasa Mankura
发明人： Naoko Seo , Shoji Kaneko , Fumi Sato , Seiji Norinobu , Mitsumasa Mankura
IPC分类号： C07J9/00
CPC分类号： C07J9/00 , C11B3/001 , C11C3/003 , C11C3/10 , C12P7/6436 , C12P7/6454 , C12P33/00
摘要： According to the present invention, there is provided a process for enzymatically producing dietary sterol fatty acid esters having physiological activities from phytosterols and fatty acids or oils and fats using lipase as a catalyst; the synthetic reaction conditions and the purification steps are so structured that dietary sterol fatty acid esters superior in sensory qualities including color, odor and taste, and safety, which is applicable as a general food, a health food or pharmaceuticals can be produced.
摘要翻译： 根据本发明，提供了一种使用脂肪酶作为催化剂从植物甾醇和脂肪酸或油和脂肪中产生具有生理活性的膳食甾醇脂肪酸酯的方法， 合成反应条件和纯化步骤如此构成，可以生产出适用于普通食品，保健食品或药物的感官质量优异的饮食甾醇脂肪酸酯，包括颜色，气味和味道以及安全性。
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公开(公告)号：US20050100994A1
公开(公告)日：2005-05-12
申请号：US10381174
申请日：2001-09-26
申请人： Naoko Seo , Shoji Kaneko , Fumi Sato , Seiji Norinobu , Mitsumasa Mankura
发明人： Naoko Seo , Shoji Kaneko , Fumi Sato , Seiji Norinobu , Mitsumasa Mankura
CPC分类号： C07J9/00 , C11B3/001 , C11C3/003 , C11C3/10 , C12P7/6436 , C12P7/6454 , C12P33/00
摘要： According to the present invention, there is provided a process for enzymatically producing dietary sterol fatty acid esters having physiological activities from phytosterols and fatty acids or oils and fats using lipase as a catalyst; the synthetic reaction conditions and the following purification steps are so structured that dietary sterol fatty acid esters superior in sensory qualities including color, odor and taste, and safety, which is applicable as a general food, a health food or pharmaceuticals can be produced. In a process for enzymatically producing physiological active dietary sterol fatty acid esters (step 124) from sterols derived from vegetable (step 100) and fatty acids (step 102) or fats and oils containing triacylglycerol as the main component (step 202) employing lipase as a catalyst, according to the present invention, the synthetic reaction of sterol fatty acid esters by the lipase is carried out for a predetermined time period in a system at a predetermined temperature and water content, enzyme inactivation treatment, dehydration treatment and enzyme protein removal treatment are carried out, and unreacted sterols and fatty acids are removed by molecular distillation treatment (step 110), coloring components are removed by treatment with an adsorbent (step 116), odorous components are removed by steam distillation treatment (step 122), and dietary sterol fatty acid esters superior in sensory properties and safety (step 124) is obtained.
摘要翻译： 根据本发明，提供了一种使用脂肪酶作为催化剂从植物甾醇和脂肪酸或油和脂肪中产生具有生理活性的膳食甾醇脂肪酸酯的方法， 合成反应条件和以下纯化步骤的结构使得可以生产出适用于普通食品，保健食品或药物的感官品质优异的饮食甾醇脂肪酸酯，包括颜色，气味和味道以及安全性。 在将来自植物甾醇（步骤100）和脂肪酸（步骤102）的甾醇或含有三酰基甘油作为主要成分的脂肪和油（步骤202）的酶促生产生理活性膳食甾醇脂肪酸酯（步骤124） 根据本发明的催化剂，通过脂肪酶的固醇脂肪酸酯的合成反应在预定温度和水含量，酶失活处理，脱水处理和酶蛋白质去除处理的系统中进行预定时间 通过分子蒸馏处理除去未反应的固醇和脂肪酸（步骤110），通过用吸附剂处理除去着色组分（步骤116），通过蒸汽蒸馏处理（步骤122）除去臭味成分，并且饮食 获得了优异的感官特性和安全性的甾醇脂肪酸酯（步骤124）。
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公开(公告)号：US06660491B2
公开(公告)日：2003-12-09
申请号：US09988919
申请日：2001-11-19
申请人： Seiji Norinobu , Naoko Seo , Fumi Sato , Shoji Kaneko , Mitsumasa Mankura
发明人： Seiji Norinobu , Naoko Seo , Fumi Sato , Shoji Kaneko , Mitsumasa Mankura
IPC分类号： C12Q144
摘要： A process for enzymatically producing, from a vegetable oil deodorizer distillate, a dietary sterol fatty acid ester superior in flavor qualities (e.g., color, odor and taste) and safety and containing no or little trans fatty acids, wherein the conditions for treatment of the starting material, synthetic reaction and subsequent purification are controlled so that the sterol fatty acid ester becomes applicable as a daily food material, a health food material or a pharmaceutical material. In the process, fatty acid esters, e.g., triacylglycerol, in the vegetable oil deodorizer distillate are previously degraded by hydrolysis with a chemical catalyst, fatty acids produced in the hydrolysis are removed by molecular distillation to give a sterol-containing fraction. The sterol-containing fraction is added with any fat and oil primarily comprising triacylglycerol. The mixture is used as the starting material, and the synthetic reaction is performed under strictly controlled conditions using a lipolytic enzyme. The resulting product undergoes several steps of purification process to provide qualities suitable for a food material.
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