公开(公告)号：US20110059433A1

公开(公告)日：2011-03-10

申请号：US11919474

申请日：2006-04-26

申请人： Frederic Marc ,  Serge Ohresser

发明人： Frederic Marc ,  Serge Ohresser

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12M1/00

CPC分类号： C12Q1/6888 ,  B01D61/18 ,  B01L3/502 ,  B01L2300/0618 ,  B01L2300/0681 ,  C12Q1/04 ,  C12Q2600/16

摘要： The present invention relates to a method for the specific detection on a filter of one or more microorganisms present in a fluid, characterized in that it comprises the following steps: a) contacting the microorganisms present in the fluid or on the surface with the filter; b) amplifying specifically the nucleic acids from the microorganism or microorganisms present on the filter, in an isothermal manner, in order to obtain amplification products, c) detecting the amplification products. The invention also relates to a device, a kit and oligonucleotides suitable for the implementation of this method.

摘要翻译： 本发明涉及用于特定检测存在于流体中的一种或多种微生物的过滤器的方法，其特征在于其包括以下步骤：a）使流体或表面上存在的微生物与过滤器接触; b）以等温方式特异性扩增存在于过滤器上的微生物或微生物的核酸，以获得扩增产物，c）检测扩增产物。 本发明还涉及适用于实施该方法的装置，试剂盒和寡核苷酸。

公开(公告)号：US20110318744A1

公开(公告)日：2011-12-29

申请号：US13195336

申请日：2011-08-01

申请人： Frederic Marc ,  Serge Ohresser

发明人： Frederic Marc ,  Serge Ohresser

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6888 ,  B01D61/18 ,  B01L3/502 ,  B01L2300/0618 ,  B01L2300/0681 ,  C12Q1/04 ,  C12Q2600/16

摘要： The present invention relates to a method for the specific detection on a filter of one or more microorganisms present in a fluid, characterized in that it comprises the following steps: a) contacting the microorganisms present in the fluid or on the surface with the filter; b) amplifying specifically the nucleic acids from the microorganism or microorganisms present on the filter, in an isothermal manner, in order to obtain amplification products, c) detecting the amplification products. The invention also relates to a device, a kit and oligonucleotides suitable for the implementation of this method.

摘要翻译： 本发明涉及用于特定检测存在于流体中的一种或多种微生物的过滤器的方法，其特征在于其包括以下步骤：a）使流体或表面上存在的微生物与过滤器接触; b）以等温方式特异性扩增存在于过滤器上的微生物或微生物的核酸，以获得扩增产物，c）检测扩增产物。 本发明还涉及适用于实施该方法的装置，试剂盒和寡核苷酸。

公开(公告)号：US07192734B2

公开(公告)日：2007-03-20

申请号：US10305831

申请日：2002-11-27

申请人： Serge Ohresser

发明人： Serge Ohresser

IPC分类号： C12Q1/04

CPC分类号： C12Q1/045

摘要： The invention relates to a method of testing for the presence of microorganisms in a gaseous environment comprising hydrogen peroxide, comprising the following steps: (i) bringing the gaseous environment comprising the hydrogen peroxide into contact with an agar growth medium, comprising a salt of pyruvic acid, (ii) placing the growth medium in an environment favoring the development of colonies of microorganisms; (iii) determining the presence of colonies of microorganisms which may have developed during step (ii). The invention also relates to a cassette containing an agar growth medium comprising a salt of pyruvic acid.

摘要翻译： 本发明涉及一种在包含过氧化氢的气体环境中测试微生物的存在的方法，包括以下步骤：（i）使包含过氧化氢的气态环境与包含丙酮酸盐的琼脂生长培养基接触 酸，（ii）将生长培养基置于有利于微生物菌落发育的环境中; （iii）确定可能在步骤（ii）期间发育的微生物菌落的存在。 本发明还涉及含有包含丙酮酸盐的琼脂生长培养基的盒。