公开(公告)号：US20220280566A1

公开(公告)日：2022-09-08

申请号：US17603937

申请日：2019-10-31

Applicant: ShanghaiTech University

Inventor： Haopeng WANG ,  Wentao LI ,  Shizhen QIU ,  Ping WEI

IPC: A61K35/17 ,  C07K14/725 ,  C07K14/705 ,  A61P35/00 ,  C12N15/62 ,  C12N5/0783 ,  C12N7/00 ,  C12N15/86

Abstract: The present invention provides a chimeric antigen receptor, which includes: an extracellular domain, a transmembrane domain, and an intracellular domain connected in sequence. The extracellular domain includes an antigen recognition region; the intracellular domain includes a costimulatory signaling region and a CD3ζ intracellular region that are connected in sequence, to form a costimulatory signaling region-CD3ζ intracellular region; and the costimulatory signaling region-CD3ζ intracellular region is a polypeptide formed by mutation of lysine in a wild-type costimulatory signaling region-CD3ζ intracellular region into arginine. The present invention provides a method for optimization and modification of CAR-T, in which all lysine sites in an intracellular segment of CAR are mutated into arginine, thereby blocking ubiquitination modification of the CAR after antigen challenge. This strategy is applicable to different CARs and changing different intracellular costimulatory domains, and in particular provides a solution to the problem of poor proliferation of CAR-T in solid tumors.

公开(公告)号：US20220401465A1

公开(公告)日：2022-12-22

申请号：US17616200

申请日：2019-06-04

Applicant: ShanghaiTech University

Inventor： Gaofeng FAN ,  Haopeng WANG ,  Yuetong WANG ,  Fei WANG

IPC: A61K31/7084 ,  A61K38/17 ,  A61K35/17 ,  A61K45/06

Abstract: Disclosed are uses of NAD+ and/or NAD+ agonists and/or NAD+ inhibitors for preparing a preparation or a kit, and a combination preparation including T cells and NAD+ and/or NAD+ agonists and/or NAD+ inhibitors. The preparation or kit is used for regulating T cell activity, regulating the expression level of CD69 on the surface of T cells, and/or regulating the phosphorylation level in T cells, and/or treating diseases related to T cell activity.