公开(公告)号：US11773145B2

公开(公告)日：2023-10-03

申请号：US16632299

申请日：2018-07-20

Applicant: ShanghaiTech University

Inventor： Jia Liu ,  Biao Jiang

IPC: C07K14/33 ,  C07K19/00 ,  A61K38/00

CPC classification number: C07K14/33 ,  C07K19/00 ,  A61K38/00 ,  C07K2319/55 ,  C07K2319/81

Abstract: Provided are chimeric polypeptides that include one or more zinc finger motifs fused to a therapeutic peptide such as botulinum neurotoxins (BoNTs). The zinc finger motif may be located at the C-terminal side of the BoNT and the chimeric polypeptide can optionally include two or more such zinc finger motifs. It said the disclosed chimeric polypeptides can be efficiently delivered to a subject transdermally.

公开(公告)号：US20210317480A1

公开(公告)日：2021-10-14

申请号：US17272252

申请日：2019-08-29

Applicant: ShanghaiTech University

Inventor： Jia Liu ,  Biao Jiang ,  Peixiang Ma ,  Guang Yang

IPC: C12N15/90 ,  A61K38/46 ,  A61K38/16 ,  C12N7/00 ,  A61K38/50 ,  A61K31/7088

Abstract: Provided are amino acid sequences capable of binding to and inhibiting a Cas protein's ability to bind to a nucleic acid molecule, thereby inhibiting the Cas protein's function in genome editing. Such Cas protein inhibitors, which can be comprised of a major coat protein (G8P), an extracellular region of the G8P (G8PEX), or a biological equivalent, are useful in improving the specificity of Cas protein-based genome editing procedures.

公开(公告)号：US20240018196A1

公开(公告)日：2024-01-18

申请号：US18451700

申请日：2023-08-17

Applicant: ShanghaiTech University

Inventor： Jia Liu ,  Biao Jiang

IPC: C07K14/33 ,  C07K19/00

CPC classification number: C07K14/33 ,  C07K19/00 ,  A61K38/00

Abstract: The present disclosure provides chimeric polypeptides that include one or more zinc finger motif fused to a therapeutic peptide such as botulinum neurotoxins (BoNTs). The zinc finger motif may be located at the C-terminal side of the BoNT and the chimeric polypeptide can optionally include two or more such zinc finger motifs. It is shown that the disclosed chimeric polypeptides can be efficiently delivered to a subject transdermally.

公开(公告)号：US20200231634A1

公开(公告)日：2020-07-23

申请号：US16632299

申请日：2018-07-20

Applicant: ShanghaiTech University

Inventor： Jia Liu ,  Biao Jiang

IPC: C07K14/33 ,  C07K19/00

Abstract: Provided are chimeric polypeptides that include one or more zinc finger motifs fused to a therapeutic peptide such as botulinum neurotoxins (BoNTs). The zinc finger motif may be located at the C-terminal side of the BoNT and the chimeric polypeptide can optionally include two or more such zinc finger motifs. It said the disclosed chimeric polypeptides can be efficiently delivered to a subject transdermally.

公开(公告)号：US11492645B2

公开(公告)日：2022-11-08

申请号：US17272252

申请日：2019-08-29

Applicant: ShanghaiTech University

Inventor： Jia Liu ,  Biao Jiang ,  Peixiang Ma ,  Guang Yang

IPC: C12N15/63 ,  A61K31/70 ,  C12N15/90 ,  A61K31/7088 ,  A61K38/16 ,  A61K38/46 ,  A61K38/50 ,  C12N7/00 ,  A01K67/00

Abstract: Provided are amino acid sequences capable of binding to and inhibiting a Cas protein's ability to bind to a nucleic acid molecule, thereby inhibiting the Cas protein's function in genome editing. Such Cas protein inhibitors, which can be comprised of a major coat protein (G8P), an extracellular region of the G8P (G8PEX), or a biological equivalent, are useful in improving the specificity of Cas protein-based genome editing procedures.

公开(公告)号：US20210207175A1

公开(公告)日：2021-07-08

申请号：US17121449

申请日：2020-12-14

Applicant: Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences ,  SHANGHAITECH UNIVERSITY

Inventor： Jia Liu ,  Jiajia Dong

IPC: C12N15/90 ,  C07D403/12 ,  C12N9/22 ,  C12N15/11

Abstract: A compound for improving the gene editing specificity and application thereof. Specifically disclosed is a compound represented by formula I or a use of a pharmaceutically acceptable salt thereof. The compound and the pharmaceutically acceptable salt thereof are used for preparing an inhibitor, a composition, or a formulation for inhibiting gene editing and/or improving the gene editing specificity. The structure of the formula I is as stated in the description. The compound can significantly improve the accuracy of CRISPR gene editing, thereby providing a simple and high-efficient policy for accurate gene editing.