公开(公告)号：US20230094769A1

公开(公告)日：2023-03-30

申请号：US17862354

申请日：2022-07-11

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Bei Yang ,  Li Yang ,  Xingxu Huang ,  Lijie Wang

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/50 ,  C12N9/78 ,  C12N15/11

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion protein may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitor, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

公开(公告)号：US11384353B2

公开(公告)日：2022-07-12

申请号：US17427040

申请日：2020-02-03

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Bei Yang ,  Li Yang ,  Xingxu Huang ,  Lijie Wang

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/50 ,  C12N15/11 ,  C12N9/78

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion proteins may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitory domain, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

公开(公告)号：US11840685B2

公开(公告)日：2023-12-12

申请号：US17862354

申请日：2022-07-11

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Bei Yang ,  Li Yang ,  Xingxu Huang ,  Lijie Wang

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/50 ,  C12N9/78 ,  C12N15/11

CPC classification number: C12N15/102 ,  C12N9/22 ,  C12N9/506 ,  C12N9/78 ,  C12N15/111 ,  C07K2319/50 ,  C07K2319/85 ,  C12N2310/20 ,  C12Y304/22044 ,  C12Y305/04004 ,  C12Y305/04005

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion protein may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitor, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

公开(公告)号：US20210163913A1

公开(公告)日：2021-06-03

申请号：US16770572

申请日：2019-02-22

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Li Yang ,  Xingxu Huang ,  Bei Yang ,  Xiao Wang ,  Jianan Li

IPC: C12N9/78 ,  C12N9/22 ,  C12N15/90 ,  C12N15/11

Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

公开(公告)号：US20240026342A1

公开(公告)日：2024-01-25

申请号：US18478861

申请日：2023-09-29

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Bei Yang ,  Li Yang ,  Xingxu Huang ,  Lijie Wang

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/50 ,  C12N9/78 ,  C12N15/11

CPC classification number: C12N15/102 ,  C12N9/22 ,  C12N9/506 ,  C12N9/78 ,  C12N15/111 ,  C12N2310/20 ,  C07K2319/50 ,  C07K2319/85 ,  C12Y304/22044 ,  C12Y305/04004 ,  C12Y305/04005

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion protein may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitor, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

公开(公告)号：US11884947B2

公开(公告)日：2024-01-30

申请号：US16770572

申请日：2019-02-22

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Li Yang ,  Xingxu Huang ,  Bei Yang ,  Xiao Wang ,  Jianan Li

IPC: C12N9/78 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12N15/01

CPC classification number: C12N9/78 ,  C12N9/22 ,  C12N15/01 ,  C12N15/907 ,  C12Y305/04 ,  C07K2319/00 ,  C12N2310/20 ,  C12N2800/80

Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

公开(公告)号：US20220064626A1

公开(公告)日：2022-03-03

申请号：US17427040

申请日：2020-02-03

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Bei Yang ,  Li Yang ,  Xingxu Huang ,  Lijie Wang

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N9/50 ,  C12N9/78

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion proteins may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitory domain, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

公开(公告)号：US12203077B2

公开(公告)日：2025-01-21

申请号：US16640337

申请日：2018-08-28

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Xingxu Huang ,  Li Yang ,  Bei Yang ,  Xiaosa Li ,  Ying Wang ,  Yajing Liu

IPC: C12N9/22 ,  C07K14/32 ,  C12N9/78 ,  C12N15/10 ,  C12N15/79

Abstract: Provided are base editors containing a cytidine and a catalytically inactive version of Lachnospiraceae bacterium Cpf1 (LbCpf1). The new base editors have greatly improved editing efficiency and fidelity as compared to Cas9-based base editors, and have different editing windows.

公开(公告)号：US20240117335A1

公开(公告)日：2024-04-11

申请号：US18525555

申请日：2023-11-30

Applicant: ShanghaiTech University

Inventor： Jia CHEN ,  Li Yang ,  Xingxu Huang ,  Bei Yang ,  Xiao Wang ,  Jianan Li

IPC: C12N9/78 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC classification number: C12N9/78 ,  C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12Y305/04 ,  C07K2319/00 ,  C12N2310/20 ,  C12N2800/80

Abstract: Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.