公开(公告)号：US08853380B2

公开(公告)日：2014-10-07

申请号：US13702028

申请日：2011-06-03

申请人： Shardul Salunkhe ,  Bhaskarjyoti Prasad ,  Praveen Muneshwar ,  Anjali Apte Deshpande ,  Sriram Padmanabhan

发明人： Shardul Salunkhe ,  Bhaskarjyoti Prasad ,  Praveen Muneshwar ,  Anjali Apte Deshpande ,  Sriram Padmanabhan

IPC分类号： C07H21/04 ,  C12N9/00 ,  C12P21/06 ,  C07K14/555 ,  C07K14/535 ,  C07K14/54 ,  C07K14/49 ,  C07K14/55 ,  C12N9/96 ,  C07K14/635 ,  C12N9/72 ,  C07K14/31 ,  C07K14/485

CPC分类号： C07K14/635 ,  C07K14/31 ,  C07K14/485 ,  C07K14/49 ,  C07K14/535 ,  C07K14/5403 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/5431 ,  C07K14/55 ,  C07K14/555 ,  C07K2319/35 ,  C07K2319/50 ,  C12N9/6459 ,  C12N9/96 ,  C12P21/06 ,  C12Y304/21069

摘要： The present invention relates to modified SAK gene having amino acid SEQ ID 2. The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which imparts improved stability to the heterologous protein of interest. Further the invention relates to process of purification of recombinant heterologous proteins from bacterial inclusion bodies using modified SAK.

摘要翻译： 本发明涉及具有氨基酸SEQ ID No.2的经修饰的SAK基因。本发明还涉及克隆和表达修饰的SAK基因融合蛋白的方法，其赋予所感兴趣的异源蛋白质更好的稳定性。 此外，本发明涉及使用改良的SAK从细菌包涵体纯化重组异源蛋白质的方法。

公开(公告)号：US20130122542A1

公开(公告)日：2013-05-16

申请号：US13702028

申请日：2011-06-03

申请人： Shardul Salunkhe ,  Bhaskarjyoti Prasad ,  Praveen Muneshwar ,  Anjali Apte Deshpande ,  Sriram Padmanabhan

发明人： Shardul Salunkhe ,  Bhaskarjyoti Prasad ,  Praveen Muneshwar ,  Anjali Apte Deshpande ,  Sriram Padmanabhan

IPC分类号： C12P21/06 ,  C12N9/96

CPC分类号： C07K14/635 ,  C07K14/31 ,  C07K14/485 ,  C07K14/49 ,  C07K14/535 ,  C07K14/5403 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/5431 ,  C07K14/55 ,  C07K14/555 ,  C07K2319/35 ,  C07K2319/50 ,  C12N9/6459 ,  C12N9/96 ,  C12P21/06 ,  C12Y304/21069

摘要： The present invention relates to modified SAK gene having amino acid SEQ ID 2. The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which imparts improved stability to the heterologous protein of interest. Further the invention relates to process of purification of recombinant heterologous proteins from bacterial inclusion bodies using modified SAK.