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公开(公告)号：USH622H
公开(公告)日：1989-04-04
申请号：US195238
申请日：1988-05-18
IPC分类号： C12Q1/50
CPC分类号： C12Q1/50
摘要： In a dry analytical element for the determination of creatine kinase enzymatic activity is disclosed, comprising at least one porous medium and at least one water permeable layer having a liquid contact relation with the porous medium, the porous medium or the water permeable layer contains creatine phosphoric acid, ADP, a thiol compound as a creatine kinase activation agent, glucose, hexokinase, G6PDH, NAD.sup.+, an electron transmission agent and a formazane dye forming tetrazolium salt, the improvement wherein at least one water permeable layer having a liquid contact relation with the porous medium contains a formazane dye forming tetrazolium salt and not more than 16 g/m.sup.2 of gelatin.
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2.依法登记的发明
公开(公告)号：USH791H
公开(公告)日：1990-06-05
申请号：US106906
申请日：1987-10-08
CPC分类号： G01N33/728
摘要： A multilayer analytical element for quantitative analysis of bilirubin in a liquid sample by a diazo method, which comprises a laminated structure consisting essentially of:(1) a porous reagent layer containing an acidic binder and a diazonium salt being reactive to bilirubin to produce azobilirubin;(2) an absorbent layer capable of absorbing an excessive liquid portion; and(3) a liquid-impermeable light-transmissive support.
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3.发明授权Analytical element having a polyacrylamide or derivative to minimize background for assaying enzyme activity 失效具有聚丙烯酰胺或衍生物的分析元件以最小化测定酶活性的背景
公开(公告)号：US5006458A
公开(公告)日：1991-04-09
申请号：US135670
申请日：1987-12-21
CPC分类号： G01N33/525 , Y10S435/805 , Y10S436/81 , Y10S436/904
摘要： A dry-type analytical element suitable for measuring enzyme activity of in a liquid sample, characterized by incorporating a polyacrylamide, polymethacrylamide or their derivatives into at least one water-permeable layer. The background concentration of the dry-type analytical element exhibits minimal increase even under a fluorescent light, and allows an accurate measured value to be easily obtained.
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4.发明授权
公开(公告)号：US4900665A
公开(公告)日：1990-02-13
申请号：US185294
申请日：1988-04-19
CPC分类号： G01N33/526 , C12Q1/42 , C12Q2334/10 , Y10S435/805
摘要： An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing one or more compounds functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.
摘要翻译： 一种用于测量碱性磷酸酶活性的整体多层分析元件，其包含含有碱性磷酸酶敏感的自显影底物的机织织物或编织物的多孔扩散层，含有一种或多种用作磷酸盐的化合物的缓冲层 酸受体和缓冲剂和层叠形式的支撑层。
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5.发明授权Dry analytical element having a spread control area for assaying enzyme activity 失效具有用于测定酶活性的扩散控制区的干燥分析元件
公开(公告)号：US4889797A
公开(公告)日：1989-12-26
申请号：US54432
申请日：1987-05-26
CPC分类号： G01N33/52 , C12Q1/32 , C12Q1/40 , C12Q1/42 , C12Q1/48 , Y10S435/805 , Y10S435/81 , Y10S436/808 , Y10S436/81
摘要： A dry analytical element for assaying an enzyme in a liquid is disclosed, comprising a support having provided thereon at least a porous liquid-spreading layer composed of water non-absorbent fibers, wherein said porous liquid-spreading layer contains a substrate for an enzyme to be assayed and a hydrophilic polymer in an amount effective to decrease a spreading area by at least 20%. The analytical element exhibits high sensitivity and high reproducibility.
摘要翻译： 公开了一种用于测定液体中的酶的干燥分析元件，其包括至少在由非水吸收性纤维构成的多孔液体扩散层上提供的支撑体，其中所述多孔液体扩散层含有用于酶的底物 并且将亲水性聚合物的量有效地将扩散面积减少至少20％。 分析元件显示出高灵敏度和高再现性。
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6.发明授权Reagent sheet and integral multilayer analytical element for measurement of GGT activity 失效用于测量GGT活性的试剂片和整体多层分析元件
公开(公告)号：US4916059A
公开(公告)日：1990-04-10
申请号：US876975
申请日：1986-06-20
申请人： Shigeki Kageyama , Harumi Katsuyama
发明人： Shigeki Kageyama , Harumi Katsuyama
CPC分类号： G01N33/526 , C12Q1/48
摘要： A reagent sheet for the measurement of GGT (.gamma.-glutamyl transferase) activity comprising a porous sheet containing a cationic surfactant and a self-developable substrate upon contact with .gamma.-glutamyl transferase. An integral multilayer analytical element for the measurement of GGT activity, comprising a porous spreading layer containing a cationic surfactant and a self-developable substrate in response to contact with .gamma.-glutamyl transferase, a water-absorbing layer and a support in a laminated form. The substrate can be either .gamma.-glutamyl-p-nitroamilide or .gamma.-glutamyl-3-carboxy-4-nitroamilide.
摘要翻译： 用于测量GGT（γ-谷氨酰转移酶）活性的试剂片，其包含含有阳离子表面活性剂的多孔片材和与γ-谷氨酰转移酶接触时的自显影底物。 用于测量GGT活性的整体多层分析元件，包括响应于与γ-谷氨酰转移酶，吸水层和层叠形式的载体接触而含有阳离子表面活性剂和自显影底物的多孔扩散层。 底物可以是γ-谷氨酰 - 对 - 硝酰基苯胺或γ-谷氨酰基-3-羧基-4-硝基己酰苯胺。
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公开(公告)号：US4902477A
公开(公告)日：1990-02-20
申请号：US273631
申请日：1988-11-17
申请人： Harumi Katsuyama , Shigeki Kageyama , Kumiko Sato
发明人： Harumi Katsuyama , Shigeki Kageyama , Kumiko Sato
CPC分类号： G01N33/728 , G01N33/526 , Y10S436/903 , Y10T436/146666
摘要： An analytical element for quantitative analysis of bilirubin in a liquid sample by a diazo method, characterized in that the bilirubin detection reagent is a non-diffusive aryldiazonium salt having in the aryl group at least one substituent selected from the group consisting of an alkoxycarbonyl group, an alkylaminosulfonyl group and an alkylaminocarbonyl group.
摘要翻译： 一种用于通过重氮法定量分析液体样品中的胆红素的分析元件，其特征在于，所述胆红素检测试剂是非扩散性芳基重氮盐，其在所述芳基中具有至少一个选自烷氧基羰基， 烷基氨基磺酰基和烷基氨基羰基。
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公开(公告)号：US08906641B2
公开(公告)日：2014-12-09
申请号：US12015151
申请日：2008-01-16
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译： 本发明的目的是提供一种能够测量高浓度（高达2500U / L）的动物样品而不稀释的方法，即使在包含动物α-淀粉样品的样品中也能够特异性地测量α-淀粉酶， 淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。 本发明提供一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯 在其还原末端，反应的pH为6以上且小于7。
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公开(公告)号：US20080182285A1
公开(公告)日：2008-07-31
申请号：US12015151
申请日：2008-01-16
IPC分类号： C12Q1/40
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译： 本发明的目的是提供一种能够测量没有稀释度的动物样品的高浓度（高达2500U / L）的方法，其甚至能够特异性地测量α-淀粉酶，即使在包含动物α-淀粉酶的样品中， 淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。 本发明提供了一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯 在其还原末端，反应的pH为6以上且小于7。
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公开(公告)号：US20080279725A1
公开(公告)日：2008-11-13
申请号：US11664284
申请日：2005-09-29
CPC分类号： G01N33/525 , B01L3/5023 , G01N31/22
摘要： The object of the invention is to provide a multilayer analytical element having a non-fibrous porous film with high film strength, which does not produce uneven coloration in the form of a white-out and which has high accuracy of analysis. The present invention provides a dry multilayer analytical element for the analysis of a liquid sample which comprises a water-non-transmitting planar support on one side of which at least one adhesion layer and a porous liquid-sample-developing layer are integrally layered in the mentioned order, wherein the porous liquid-sample-developing layer comprises a non-fibrous porous film whose bending fracture strength is 20 g weight or more and whose elongation ratio when pulled with a 50-g weight is 2% or less, and wherein a adhesion layer polymer binder is caused to become seeped up in the non-fibrous porous film to 2 to 50 μm.
摘要翻译： 本发明的目的是提供一种具有高膜强度的非纤维多孔膜的多层分析元件，其不会产生白色形式的不均匀着色，并且分析精度高。 本发明提供了一种用于分析液体样品的干燥多层分析元件，该液体样品在其一侧包括水不透过平面支撑体，其中至少一个粘附层和多孔液体样品显影层一体地层叠在 其中多孔液体样品显影层包括弯曲断裂强度为20g或更大的非纤维多孔膜，其拉伸50g时的伸长率为2％以下，其中， 使粘合层聚合物粘合剂在非纤维多孔膜中渗透至2〜50μm。
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