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公开(公告)号：US08906641B2
公开(公告)日：2014-12-09
申请号：US12015151
申请日：2008-01-16
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译： 本发明的目的是提供一种能够测量高浓度（高达2500U / L）的动物样品而不稀释的方法，即使在包含动物α-淀粉样品的样品中也能够特异性地测量α-淀粉酶， 淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。 本发明提供一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯 在其还原末端，反应的pH为6以上且小于7。
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公开(公告)号：US20080182285A1
公开(公告)日：2008-07-31
申请号：US12015151
申请日：2008-01-16
IPC分类号： C12Q1/40
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译： 本发明的目的是提供一种能够测量没有稀释度的动物样品的高浓度（高达2500U / L）的方法，其甚至能够特异性地测量α-淀粉酶，即使在包含动物α-淀粉酶的样品中， 淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。 本发明提供了一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯 在其还原末端，反应的pH为6以上且小于7。
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公开(公告)号：US20090246811A1
公开(公告)日：2009-10-01
申请号：US12410035
申请日：2009-03-24
IPC分类号： C12Q1/44
CPC分类号： C12Q1/44 , G01N2333/92
摘要： The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid which contains triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, and comprises a water-impermeable support and at least one spreading or reagent layer, said method comprising the step of coating an emulsion/dispersion solution of triglyceride with an average particle size of 1 μm or less.
摘要翻译： 本发明提供了含有含有碳原子数12〜22的长链烷基脂肪酸甘油三酯，单甘油酯脂肪酶，甘油测定试剂的体液中所含的胰脂肪酶的测定用干燥分析元件的制造方法， 水不渗透性载体和至少一种扩散剂或试剂层，所述方法包括涂覆平均粒度为1μm以下的甘油三酯的乳液/分散液的步骤。
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公开(公告)号：US08168406B2
公开(公告)日：2012-05-01
申请号：US12410035
申请日：2009-03-24
CPC分类号： C12Q1/44 , G01N2333/92
摘要： The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid which contains triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, and comprises a water-impermeable support and at least one spreading or reagent layer, said method comprising the step of coating an emulsion/dispersion solution of triglyceride with an average particle size of 1 μm or less.
摘要翻译： 本发明提供了含有含有碳原子数12〜22的长链烷基脂肪酸甘油三酯，单甘油酯脂肪酶，甘油测定试剂的体液中所含的胰脂肪酶的测定用干燥分析元件的制造方法， 水不渗透性支持体和至少一种扩散剂或试剂层，所述方法包括涂布平均粒径为1μm以下的甘油三酯的乳液/分散液的步骤。
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公开(公告)号：USH622H
公开(公告)日：1989-04-04
申请号：US195238
申请日：1988-05-18
IPC分类号： C12Q1/50
CPC分类号： C12Q1/50
摘要： In a dry analytical element for the determination of creatine kinase enzymatic activity is disclosed, comprising at least one porous medium and at least one water permeable layer having a liquid contact relation with the porous medium, the porous medium or the water permeable layer contains creatine phosphoric acid, ADP, a thiol compound as a creatine kinase activation agent, glucose, hexokinase, G6PDH, NAD.sup.+, an electron transmission agent and a formazane dye forming tetrazolium salt, the improvement wherein at least one water permeable layer having a liquid contact relation with the porous medium contains a formazane dye forming tetrazolium salt and not more than 16 g/m.sup.2 of gelatin.
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6.依法登记的发明
公开(公告)号：USH791H
公开(公告)日：1990-06-05
申请号：US106906
申请日：1987-10-08
CPC分类号： G01N33/728
摘要： A multilayer analytical element for quantitative analysis of bilirubin in a liquid sample by a diazo method, which comprises a laminated structure consisting essentially of:(1) a porous reagent layer containing an acidic binder and a diazonium salt being reactive to bilirubin to produce azobilirubin;(2) an absorbent layer capable of absorbing an excessive liquid portion; and(3) a liquid-impermeable light-transmissive support.
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公开(公告)号：US20080279725A1
公开(公告)日：2008-11-13
申请号：US11664284
申请日：2005-09-29
CPC分类号： G01N33/525 , B01L3/5023 , G01N31/22
摘要： The object of the invention is to provide a multilayer analytical element having a non-fibrous porous film with high film strength, which does not produce uneven coloration in the form of a white-out and which has high accuracy of analysis. The present invention provides a dry multilayer analytical element for the analysis of a liquid sample which comprises a water-non-transmitting planar support on one side of which at least one adhesion layer and a porous liquid-sample-developing layer are integrally layered in the mentioned order, wherein the porous liquid-sample-developing layer comprises a non-fibrous porous film whose bending fracture strength is 20 g weight or more and whose elongation ratio when pulled with a 50-g weight is 2% or less, and wherein a adhesion layer polymer binder is caused to become seeped up in the non-fibrous porous film to 2 to 50 μm.
摘要翻译： 本发明的目的是提供一种具有高膜强度的非纤维多孔膜的多层分析元件，其不会产生白色形式的不均匀着色，并且分析精度高。 本发明提供了一种用于分析液体样品的干燥多层分析元件，该液体样品在其一侧包括水不透过平面支撑体，其中至少一个粘附层和多孔液体样品显影层一体地层叠在 其中多孔液体样品显影层包括弯曲断裂强度为20g或更大的非纤维多孔膜，其拉伸50g时的伸长率为2％以下，其中， 使粘合层聚合物粘合剂在非纤维多孔膜中渗透至2〜50μm。
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公开(公告)号：US5603898A
公开(公告)日：1997-02-18
申请号：US993964
申请日：1992-12-17
申请人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
发明人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
CPC分类号： G01N33/581 , G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.
摘要翻译： 一种用于免疫测定的干式分析元件，其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层，其包含（A）水溶性大分子底物和（B）与抗体结合的酶 与能够作用于上述水溶性大分子底物的样品中的配体反应。 上述分析元素还包含（C）在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗原。 本发明可以进行高灵敏度和简单的酶免疫测定。
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公开(公告)号：US5161167A
公开(公告)日：1992-11-03
申请号：US717046
申请日：1991-06-18
CPC分类号： H01S5/32325 , H01S5/2231 , H01S5/2206 , H01S5/2207 , H01S5/2209 , H01S5/221 , H01S5/2219
摘要： A semiconductor laser producing visible light includes a first conductivity type GaAs substrate, a first conductivity type (Al.sub.w Ga.sub.1-w).sub.0.5 In.sub.0.5 P cladding layer disposed on the substrate, a (Al.sub.z Ga.sub.1-z).sub.0.5 In.sub.0.5 P active layer disposed on the first conductivity type cladding layer wherein 0.ltoreq.z
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10.发明授权Analytical element having a polyacrylamide or derivative to minimize background for assaying enzyme activity 失效具有聚丙烯酰胺或衍生物的分析元件以最小化测定酶活性的背景
公开(公告)号：US5006458A
公开(公告)日：1991-04-09
申请号：US135670
申请日：1987-12-21
CPC分类号： G01N33/525 , Y10S435/805 , Y10S436/81 , Y10S436/904
摘要： A dry-type analytical element suitable for measuring enzyme activity of in a liquid sample, characterized by incorporating a polyacrylamide, polymethacrylamide or their derivatives into at least one water-permeable layer. The background concentration of the dry-type analytical element exhibits minimal increase even under a fluorescent light, and allows an accurate measured value to be easily obtained.
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