公开(公告)号：US20090042295A1

公开(公告)日：2009-02-12

申请号：US11889067

申请日：2007-08-08

申请人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

发明人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

IPC分类号： C12N5/06

CPC分类号： C12N5/0068 ,  C08G2261/3424 ,  C08L65/04 ,  C12N2533/30

摘要： A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.

摘要翻译： 提供耐久性且能够以低成本容易地以商业规模生产的细胞培养基材及其制造方法。 细胞培养基材包括基材和通过表面改性形成的层，其包含通过使由下式（II）表示的聚合物反应而生成的含氨基的聚合物：（其中n为0或正整数，m为 正整数，代表聚合度的n和m）通过化学气相沉积由下式（I）表示的甲酰基[2.2]对环烷烃形成：（其中k为0或1）与具有至少一个氨基的聚合物 -NH2）能够在其单体中形成席夫碱。 细胞培养基质的制造方法包括在基材上合成这样的聚合物的工序。

公开(公告)号：US08383409B2

公开(公告)日：2013-02-26

申请号：US13020193

申请日：2011-02-03

申请人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

发明人： Shin Ohya ,  Takashi Inoue ,  Takatoki Yamamoto ,  Teruo Fujii ,  Yasuyuki Sakai ,  Masaki Nishikawa ,  Hitomi Sakai ,  Hirosuke Naruto

IPC分类号： C12N5/02

CPC分类号： C12N5/0068 ,  C08G2261/3424 ,  C08L65/04 ,  C12N2533/30

摘要： The invention is directed to methods for the propagation or cultivation of cells including preparing a cell culture substrate, wherein the cell culture substrate includes a substrate and a layer formed by surface modification. The layer includes a polymer containing an amino group. The polymer is produced by reacting a polymer represented by formula (II): with a polymer having at least one amino group, —NH2, capable of forming a Schiff base in a monomer of formula (II), thereby forming a polymer layer constituting the layer formed by surface modification. “n” in Formula (II) is 0 or a positive integer, and m is a positive integer. n and m represent the degree of polymerization. Formula (II) is formed by chemical vapor deposition of formyl[2.2]paracyclophane. The methods further include providing cells in a medium; inoculating the cells onto the cell culture substrate; and culturing the cells, wherein the cells adhere to the cell culture substrate.

摘要翻译： 本发明涉及细胞繁殖或培养方法，包括制备细胞培养基质，其中细胞培养基质包括基质和通过表面改性形成的层。 该层包括含有氨基的聚合物。 该聚合物通过使由式（II）表示的聚合物与具有至少一个氨基的聚合物-NH 2反应生成，该聚合物能够在式（II）的单体中形成席夫碱，从而形成构成 通过表面改性形成的层。 式（II）中的n为0或正整数，m为正整数。 n和m表示聚合度。 式（II）由甲酰基[2.2]对环磷酰胺的化学气相沉积形成。 所述方法还包括在培养基中提供细胞; 将细胞接种到细胞培养基底上; 并培养细胞，其中细胞粘附于细胞培养基质。