公开(公告)号：US09637732B2

公开(公告)日：2017-05-02

申请号：US13824087

申请日：2011-11-04

申请人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Koji Tanabe ,  Hong Hyenjong

发明人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Koji Tanabe ,  Hong Hyenjong

IPC分类号： C12N15/00 ,  C12N15/01 ,  C12N5/074 ,  C12N15/86 ,  C12N9/18 ,  C12N9/12

CPC分类号： C12N15/01 ,  C12N5/0696 ,  C12N9/12 ,  C12N9/18 ,  C12N15/86 ,  C12N2501/405 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/999

摘要： Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.

公开(公告)号：US20130183757A1

公开(公告)日：2013-07-18

申请号：US13824087

申请日：2011-11-04

申请人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Koji Tanabe ,  Hong Hyenjong

发明人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Koji Tanabe ,  Hong Hyenjong

IPC分类号： C12N15/01

CPC分类号： C12N15/01 ,  C12N5/0696 ,  C12N9/12 ,  C12N9/18 ,  C12N15/86 ,  C12N2501/405 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/999

摘要： Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.

摘要翻译： 本发明提供了提高iPS细胞建立效率的方法，其包括在核苷酸步骤中使从属于细胞周期蛋白D家族的蛋白质中选出的一个或多个因子和编码其的核酸与体细胞接触 体细胞重编程。 还提供了一种生产iPS细胞的方法，包括使因子和核重编程物质与体细胞接触的步骤，iPS细胞包含编码属于细胞周期蛋白D家族的蛋白质的核酸 可以通过生产iPS细胞的方法获得，以及通过强迫iPS细胞分化的体细胞产生的方法。