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公开(公告)号：US09637732B2
公开(公告)日：2017-05-02
申请号：US13824087
申请日：2011-11-04
CPC分类号： C12N15/01 , C12N5/0696 , C12N9/12 , C12N9/18 , C12N15/86 , C12N2501/405 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/999
摘要： Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.
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公开(公告)号：US20130183757A1
公开(公告)日：2013-07-18
申请号：US13824087
申请日：2011-11-04
IPC分类号： C12N15/01
CPC分类号： C12N15/01 , C12N5/0696 , C12N9/12 , C12N9/18 , C12N15/86 , C12N2501/405 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/999
摘要： Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.
摘要翻译： 本发明提供了提高iPS细胞建立效率的方法，其包括在核苷酸步骤中使从属于细胞周期蛋白D家族的蛋白质中选出的一个或多个因子和编码其的核酸与体细胞接触 体细胞重编程。 还提供了一种生产iPS细胞的方法，包括使因子和核重编程物质与体细胞接触的步骤，iPS细胞包含编码属于细胞周期蛋白D家族的蛋白质的核酸 可以通过生产iPS细胞的方法获得，以及通过强迫iPS细胞分化的体细胞产生的方法。
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公开(公告)号：US09506039B2
公开(公告)日：2016-11-29
申请号：US13991341
申请日：2011-12-02
CPC分类号： C12N5/0696 , C12N15/85 , C12N2501/115 , C12N2501/415 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/998 , C12N2506/1307 , C12N2510/00
摘要： The invention provides a method of improving the efficiency of establishment of induced pluripotent stem cells by increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The invention also provides a method of producing induced pluripotent stem cells by contacting a somatic cell with a nuclear reprogramming substance and one or more of such proteins and nucleic acids that encode such proteins. The invention further provides an induced pluripotent stem cell that has an exogenous nucleic acid encoding such a protein, as well as agents for use in the aforesaid methods.
摘要翻译： 本发明提供了一种通过在体细胞核重编程步骤中增加一种或多种蛋白质的活化形式的水平来提高诱导多能干细胞的建立效率的方法，该蛋白质选自Ras家族成员，PI3激酶 ，RalGEF，Raf，AKT家族成员，Rheb，TCL1和S6K。 本发明还提供了通过使体细胞与核重编程物质和一种或多种这样的蛋白质和编码这种蛋白质的核酸接触来产生诱导性多能干细胞的方法。 本发明还提供了具有编码这种蛋白质的外源核酸的诱导多能干细胞，以及用于上述方法的试剂。
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公开(公告)号：US20130267030A1
公开(公告)日：2013-10-10
申请号：US13991341
申请日：2011-12-02
CPC分类号： C12N5/0696 , C12N15/85 , C12N2501/115 , C12N2501/415 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/998 , C12N2506/1307 , C12N2510/00
摘要： The invention provides a method of improving the efficiency of establishment of induced pluripotent stem cells by increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The invention also provides a method of producing induced pluripotent stem cells by contacting a somatic cell with a nuclear reprogramming substance and one or more of such proteins and nucleic acids that encode such proteins. The invention further provides an induced pluripotent stem cell that has an exogenous nucleic acid encoding such a protein, as well as agents for use in the aforesaid methods.
摘要翻译： 本发明提供了一种通过在体细胞核重编程步骤中增加一种或多种蛋白质的活化形式的水平来提高诱导多能干细胞的建立效率的方法，该蛋白质选自Ras家族成员，PI3激酶 ，RalGEF，Raf，AKT家族成员，Rheb，TCL1和S6K。 本发明还提供了通过使体细胞与核重编程物质和一种或多种这样的蛋白质和编码这种蛋白质的核酸接触来产生诱导性多能干细胞的方法。 本发明还提供了具有编码这种蛋白质的外源核酸的诱导多能干细胞，以及用于上述方法的试剂。
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公开(公告)号：US08993329B2
公开(公告)日：2015-03-31
申请号：US13393158
申请日：2010-09-22
申请人： Shinya Yamanaka , Koji Tanabe
发明人： Shinya Yamanaka , Koji Tanabe
CPC分类号： C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/608 , C12N2510/00
摘要： Provided are a method of improving iPS cell establishment efficiency, comprising the step of transferring Lin28B or a nucleic acid that encodes Lin28B to a somatic cell, particularly to a somatic cell on which Lin28 is ineffective or less effective than Lin28B in improving iPS cell establishment efficiency, and a method of producing an iPS cell, comprising the step of transferring Lin28B or a nucleic acid that encodes Lin28B and a nuclear reprogramming substance to a somatic cell. Also provided are an iPS cell comprising a nucleic acid that encodes Lin28B, that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate into a somatic cell.
摘要翻译： 提供一种提高iPS细胞建立效率的方法，其包括将Lin28B或编码Lin28B的核酸转移到体细胞，特别是Lin28在改善iPS细胞建立效率方面无效或低于Lin28B的体细胞的步骤 以及生产iPS细胞的方法，包括将Lin28B或编码Lin28B和核重编程物质的核酸转移到体细胞的步骤。 还提供了包含编码Lin28B的核酸的iPS细胞，其可以通过制备iPS细胞的方法获得，以及通过强迫iPS细胞分化成体细胞的体细胞产生的方法。
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公开(公告)号：US20110250692A1
公开(公告)日：2011-10-13
申请号：US13059188
申请日：2009-10-30
申请人： Shinya Yamanaka , Koji Tanabe
发明人： Shinya Yamanaka , Koji Tanabe
CPC分类号： C12N5/0696 , C12N2501/603 , C12N2501/605 , C12N2501/608 , C12N2501/727 , C12N2510/00
摘要： The present invention relates to a method for producing mammalian induced pluripotent stem cells, comprising introducing mammal-derived reprogramming factors comprising Oct3/4 and Nanog, or nucleic acids encoding Oct3/4 and Nanog, into mammal-derived somatic cells and thereby inducing induced pluripotent stem cells from the somatic cells, wherein the reprogramming factors comprise neither Sox2 nor nucleic acid encoding Sox2.
摘要翻译： 本发明涉及一种用于产生哺乳动物诱导的多能干细胞的方法，其包括将包含Oct3 / 4和Nanog的哺乳动物衍生的重编程因子或编码Oct3 / 4和Nanog的核酸引入哺乳动物衍生的体细胞，从而诱导诱导的多能干细胞 来自体细胞的干细胞，其中重编程因子既不包括Sox2也不包含编码Sox2的核酸。
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公开(公告)号：US20120214243A1
公开(公告)日：2012-08-23
申请号：US13393158
申请日：2010-09-22
申请人： Shinya Yamanaka , Koji Tanabe
发明人： Shinya Yamanaka , Koji Tanabe
CPC分类号： C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/608 , C12N2510/00
摘要： Provided are a method of improving iPS cell establishment efficiency, comprising the step of transferring Lin28B or a nucleic acid that encodes Lin28B to a somatic cell, particularly to a somatic cell on which Lin28 is ineffective or less effective than Lin28B in improving iPS cell establishment efficiency, and a method of producing an iPS cell, comprising the step of transferring Lin28B or a nucleic acid that encodes Lin28B and a nuclear reprogramming substance to a somatic cell. Also provided are an iPS cell comprising a nucleic acid that encodes Lin28B, that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate into a somatic cell.
摘要翻译： 提供一种提高iPS细胞建立效率的方法，其包括将Lin28B或编码Lin28B的核酸转移到体细胞，特别是Lin28在改善iPS细胞建立效率方面无效或低于Lin28B的体细胞的步骤 以及生产iPS细胞的方法，包括将Lin28B或编码Lin28B和核重编程物质的核酸转移到体细胞的步骤。 还提供了包含编码Lin28B的核酸的iPS细胞，其可以通过制备iPS细胞的方法获得，以及通过强迫iPS细胞分化成体细胞的体细胞产生的方法。
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公开(公告)号：US12036245B2
公开(公告)日：2024-07-16
申请号：US17292298
申请日：2019-10-04
申请人： Koji Tanabe , I Peace, Inc.
发明人： Koji Tanabe , Kenta Suto
IPC分类号： A61K35/545 , A61K8/98 , A61P17/14 , A61Q19/08
CPC分类号： A61K35/545 , A61K8/982 , A61P17/14 , A61Q19/08
摘要： Provided are a pharmaceutical composition or a pharmaceutical composition raw material, which contains supernatant from a culture medium used during culture to induce pluripotent stem cells.
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公开(公告)号：US11518974B2
公开(公告)日：2022-12-06
申请号：US15756031
申请日：2016-08-31
申请人： I PEACE, INC. , Koji Tanabe
发明人： Koji Tanabe , Brendan Kelly , Kenta Suto , Hidenori Shimoda , Ryoji Hiraide
IPC分类号： C12N5/074 , C12M1/36 , C12M1/00 , C12M1/34 , C12M1/33 , C12M1/42 , C12N5/078 , C12M3/00 , C12M1/02 , C12M1/12
摘要： A stem cell production system provided with a preintroduction cell-feeding solution channel 20 through which a solution containing cells passes, an induction factor-feeding solution mechanism 21 for feeding a pluripotency induction factor to the preintroduction cell-feeding solution channel 20, a factor introduction device 30 connected to the preintroduction cell-feeding solution channel 20 for making cells with induction factor introduced by introducing the pluripotency induction factor into the cells, a cell mass-making device 40 for making multiple cell masses comprising stem cells by culturing the cells with induction factor introduced, and a packaging device 100 for sequentially packaging each of the multiple cell masses.
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公开(公告)号：US08508492B2
公开(公告)日：2013-08-13
申请号：US12768203
申请日：2010-04-27
IPC分类号： G06F3/041
CPC分类号： G06F3/045
摘要： In a touch panel, one of conductive layers on a bottom of an upper substrate and on a top of a lower substrate is formed of belt-shaped conductive layers. The other is a single conductive layer facing the belt-shaped conductive layers. The single conductive layer is provided with a pair of electrodes in positions corresponding to both ends in a direction where the belt-shaped conductive layers extend. When the upper substrate is pressed while a voltage is applied between the pair of electrodes, the top and lower conductive layers contact with each other. Then, a voltage value corresponding to the pressed position in the direction where the belt-shaped conductive layers extend is generated from any of the belt-shaped conductive layers. From the voltage value and the position of the belt-shaped conductive layer from which the voltage value is generated, a pressed position on the upper substrate can be detected.
摘要翻译： 在触摸面板中，上基板的底部和下基板的顶部上的导电层中的一个由带状导电层形成。 另一个是面向带状导电层的单个导电层。 单个导电层在带状导电层延伸的方向上的两端的位置处设置有一对电极。 当在一对电极之间施加电压的同时按压上基板时，上导电层和下导电层彼此接触。 然后，从任何带状导电层产生与带状导电层延伸的方向上的按压位置对应的电压值。 根据产生电压值的带状导电层的电压值和位置，可以检测上基板上的按压位置。
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