公开(公告)号：US20230400394A1

公开(公告)日：2023-12-14

申请号：US18249325

申请日：2021-10-19

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： David Ledden ,  Jeffrey Mayfield ,  Hollie Huff

IPC: G01N1/40

CPC classification number: G01N1/4005 ,  G01N2001/4038

Abstract: A method of increasing a concentration of an antigen of a respiratory virus is provided. The method includes preparing a sample containing a first concentration of the antigen; contacting the sample containing the first concentration of the antigen to a first material having a negatively-charged surface, thereby capturing an amount of the antigen with the first material; contacting a second material to the first material to transfer the antigen from the first material to the second material, the second material having an ionic strength sufficient to release the captured antigen from the first material into the second material; and obtaining a resulting solution containing the antigen in a second concentration. The second concentration of the antigen in the resulting solution is higher than the first concentration of the antigen in the sample. Numerous other aspects in accordance with this and other embodiments are provided.

公开(公告)号：US20210389307A1

公开(公告)日：2021-12-16

申请号：US17279958

申请日：2019-09-20

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Julie Chaney ,  Jon Stradinger ,  Aaron Kauffmann ,  Jeffrey Mayfield ,  Chris Zimmerle

IPC: G01N33/53 ,  G01N21/27 ,  G01N21/78 ,  G01N21/82 ,  G01N33/543

Abstract: Devices and methods for the hook effect detection associated with analytes of interest in the conductance of one or more diagnostic assays, including, without limitation, immunoassays.

公开(公告)号：US20220143604A1

公开(公告)日：2022-05-12

申请号：US17593310

申请日：2020-03-12

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Aaron Kauffmann ,  Jeffrey Mayfield ,  David Ledden

IPC: B01L3/00

Abstract: Microfluidic devices, method for making and using same are disclosed. The microfluidic device can be made by applying a reagent in liquid form into a sample treatment site within a substrate base. The substrate base has hydrophilic properties bordering a flow channel, and has an application site, the sample treatment site and an analytical site. The flow channel is sized and configured so as to draw a sample applied into the application site to the analytical site through the sample treatment site by capillary action. The reagent is freeze-dried within the sample treatment site.