公开(公告)号：US20180306815A1

公开(公告)日：2018-10-25

申请号：US15524817

申请日：2015-11-17

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Krastyu Ugrinov ,  Lloyd Schulman ,  David Ledden ,  Janine Cox ,  Anne Novamo

IPC: G01N33/76 ,  G01N33/53 ,  G01N33/558 ,  C12M1/34 ,  G01N30/90 ,  G01N33/532

CPC classification number: G01N33/76 ,  C12M1/34 ,  G01N30/90 ,  G01N33/5306 ,  G01N33/532 ,  G01N33/558

Abstract: In one aspect, the inventive concepts disclosed herein are directed to a lateral flow assay comprising: one or more chromatographic substrates, a sample application site, a first capture site, and a second capture site. The first capture site comprising an anti-hCG capture antibody with broad affinity for a group of multiple hCG variants and the second capture site comprising an anti-hCG capture antibody that is specific to a first variant of hCG. Additional aspects of the invention disclose using the later flow assay to determine a ratio of the first variant of hCG to the group of multiple hCG variants.